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首页> 外文期刊>Molecular and Cellular Biology >The src protein contains multiple domains for specific attachment to membranes.
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The src protein contains multiple domains for specific attachment to membranes.

机译：src蛋白包含多个域，可特异性附着在膜上。

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The proteins encoded by the oncogene v-src and its cellular counterpart c-src (designated generically here as pp60src) are tightly associated with both plasma membranes and intracellular membranes. This association is due in part to the amino-terminal myristylation of pp60src, but several lines of evidence suggest that amino-terminal portions of the protein itself are also involved. We now report that pp60src contains at least three domains which, in conjunction with myristylation, are capable of mediating attachment to membranes and determining subcellular localization. We identified these domains by fusing various portions of pp60src to pyruvate kinase, which is normally a cytoplasmic protein. Amino acids 1 to 14 of pp60src are sufficient to mediate both myristylation and the attachment of pyruvate kinase to cytoplasmic granules. In contrast, amino acids 38 to 111 mediate association with the plasma membrane and perinuclear membranes, whereas amino acids 204 to 259 mediate association primarily with perinuclear membranes. We conclude that pp60src contains independent domains that target the protein to distinctive subcellular locations and thus may facilitate diverse biological functions of the protein.
机译：由癌基因v-src及其细胞对应物c-src（此处统称为pp60src）编码的蛋白质与质膜和细胞内膜都紧密相关。这种关联部分是由于pp60src的氨基末端肉豆蔻酰化引起的，但几条证据表明，蛋白质本身的氨基末端部分也参与其中。我们现在报告pp60src包含至少三个域，与肉豆蔻酰化结合，能够介导膜的附着并确定亚细胞定位。我们通过将pp60src的各个部分融合到丙酮酸激酶（通常是细胞质蛋白）中来识别这些域。 pp60src的1至14位氨基酸足以介导肉豆蔻酰化和丙酮酸激酶与细胞质颗粒的结合。相反，氨基酸38至111介导与质膜和核周膜的缔合，而氨基酸204至259主要介导与核周膜的缔合。我们得出的结论是，pp60src包含将蛋白质靶向独特的亚细胞位置的独立域，因此可以促进蛋白质的多种生物学功能。

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