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首页> 外文期刊>Molecular and Cellular Biology >Sequencing of Saccharomyces telomeres cloned using T4 DNA polymerase reveals two domains.
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Sequencing of Saccharomyces telomeres cloned using T4 DNA polymerase reveals two domains.

机译：使用T4 DNA聚合酶克隆的酿酒酵母端粒的测序揭示了两个域。

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摘要

By using T4 DNA polymerase rather than S1 or Bal31 nuclease to clone yeast telomeres, very little telomeric DNA is lost. These clones were used to determine the DNA sequence of virtually the entire telomeric tract. Our results demonstrated that a slightly modified version, C2-3A(CA)1-6, of the consensus derived from sequence analysis of more-internal regions (J. Shampay, J. W. Szostak, and E. H. Blackburn, Nature [London] 310:154-157, 1984) extends to the very end of the chromosome. The sequence analysis also suggests that yeast telomeres consist of two domains: the proximal 120 to 150 base pairs, which appear to be protected from processes such as recombination, degradation, and elongation, and the distal portion of the telomere, which is more susceptible to these events.
机译：通过使用T4 DNA聚合酶而不是S1或Bal31核酸酶克隆酵母端粒，几乎不会丢失端粒DNA。这些克隆被用于确定实际上整个端粒束的DNA序列。我们的结果表明，从内部区域的序列分析（J. Shampay，JW Szostak和EH Blackburn，自然[伦敦] 310：154）得出的共识的略微修改版本C2-3A（CA）1-6 -157，1984）延伸到染色体的最末端。序列分析还表明，酵母端粒由两个结构域组成：近端120至150个碱基对，似乎受到保护，免受重组，降解和延伸等过程的侵害，而端粒的远端部分更易于感染这些事件。

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