In vitro studies of the binding of the ARGR proteins to the ARG5,6 promoter.

E Dubois; F Messenguy

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In vitro studies of the binding of the ARGR proteins to the ARG5,6 promoter.

机译：ARGR蛋白与ARG5,6启动子结合的体外研究。

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ARGRI, ARGRII, and ARGRIII regulatory proteins control the expression of arginine anabolic and catabolic genes in Saccharomyces cerevisiae. We show here that they are also required in vitro to observe a protein-DNA complex with the promoter of the ARG5,6 gene. The specific binding of ARGR proteins in vitro is stimulated by arginine. Antibodies raised against a synthetic MCM1 polypeptide retard the migration of ARGR-DNA complex on gel mobility shift assays. This result suggests that MCM1 could be an additional regulatory element of arginine metabolism.

机译：ARGRI，ARGRII和ARGRIII调节蛋白控制酿酒酵母中精氨酸合成代谢和分解代谢基因的表达。我们在这里显示，它们还需要在体外观察具有ARG5,6基因启动子的蛋白质-DNA复合物。精氨酸刺激体外ARGR蛋白的特异性结合。针对合成MCM1多肽产生的抗体可在凝胶迁移率变动分析中阻止ARGR-DNA复合物的迁移。该结果表明，MCM1可能是精氨酸代谢的另一个调控元素。

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《Molecular and Cellular Biology》 |1991年第4期|共7页
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E Dubois; F Messenguy;
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中图分类细胞生物学;
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In vitro studies of the binding of the ARGR proteins to the ARG5,6 promoter.

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