Epidermal growth factor (EGF) stimulates association and kinase activity of Raf-1 with the EGF receptor.

H App; R Hazan; A Zilberstein; A Ullrich; J Schlessinger; U Rapp

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Epidermal growth factor (EGF) stimulates association and kinase activity of Raf-1 with the EGF receptor.

机译：表皮生长因子（EGF）刺激Raf-1与EGF受体的缔合和激酶活性。

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Raf-1 serine- and threonine-specific protein kinase is transiently activated in cells expressing the epidermal growth factor (EGF) receptor upon treatment with EGF. The stimulated EGF receptor coimmunoprecipitates with Raf-1 kinase and mediates protein kinase C-independent phosphorylation of Raf-1 on serine residues. Hyperphosphorylated Raf-1 has lower mobility on sodium dodecyl sulfate gels and has sixfold-increased activity in immunocomplex kinase assay with histone H1 or Raf-1 sequence-derived peptide as a substrate. Raf-1 activation requires kinase-active EGF receptor; a point mutant lacking tyrosine kinase activity in inactive in Raf-1 coupling and association. It is noteworthy that tyrosine phosphorylation of c-Raf-1 induced by EGF was not detected in these cells. These observations suggest that Raf-1 kinase may act as an important downstream effector of EGF signal transduction.

机译：用表皮生长因子（EGF）处理后，Raf-1丝氨酸和苏氨酸特异性蛋白激酶在表达表皮生长因子（EGF）受体的细胞中被瞬时激活。刺激的EGF受体与Raf-1激酶共免疫沉淀，并介导丝氨酸残基上Raf-1的蛋白激酶C依赖性磷酸化。高磷酸化的Raf-1在十二烷基硫酸钠凝胶上的迁移率较低，并且在以组蛋白H1或Raf-1序列衍生的肽为底物的免疫复合激酶测定中，其活性增加了六倍。 Raf-1激活需要激酶活性EGF受体;缺乏酪氨酸激酶活性的点突变体，在Raf-1偶联和缔合中无效。值得注意的是，在这些细胞中未检测到由EGF诱导的c-Raf-1的酪氨酸磷酸化。这些观察结果表明，Raf-1激酶可能是EGF信号转导的重要下游效应子。

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《Molecular and Cellular Biology》 |1991年第2期|共7页
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H App; R Hazan; A Zilberstein; A Ullrich; J Schlessinger; U Rapp;
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中图分类细胞生物学;
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1. Epidermal growth factor (EGF) promotes phosphorylation at threonine-654 of the EGF receptor: possible role of protein kinase C in homologous regulation of the EGF receptor. [J] . B Whiteley, L Glaser Journal of cell biology . 1986,第4期

机译：表皮生长因子（EGF）促进EGF受体苏氨酸654处的磷酸化：蛋白激酶C在EGF受体同源调节中的可能作用。
2. Rapid uptake of tyrphostin into A431 human epidermoid cells is followed by delayed inhibition of epidermal growth factor (EGF)-stimulated EGF receptor tyrosine kinase activity. [J] . C A Faaland, F H Mermelstein, J Hayashi, Molecular and Cellular Biology . 1991,第5期

机译：在A431人表皮样细胞中快速摄取酪氨酸抑制蛋白，然后延迟抑制表皮生长因子（EGF）刺激的EGF受体酪氨酸激酶活性。
3. Role of G protein-coupled receptors (GPCR), matrix metalloproteinases 2 and 9 (MMP2 and MMP9), heparin-binding epidermal growth factor-like growth factor (hbEGF), epidermal growth factor receptor (EGFR), erbB2, and insulin-like growth factor 1 receptor (IGF-1R) in trenbolone acetate-stimulated bovine satellite cell proliferation [J] . Journal of Animal Science . 2015,第9期

机译：G蛋白偶联受体（GPCR），基质金属蛋白酶2和9（MMP2和MMP9）的作用，肝素结合表皮生长因子样生长因子（HBEGF），表皮生长因子受体（EGFR），ERBB2和胰岛素样生长因子1受体（IGF-1R）在醋酸甲醇刺激的牛卫星细胞增殖中
4. Injury and stimulation of Porcine Aortic Endothelial cells (PAE) with nucleotides or EGF result in differential phosphorylation of epidermal growth factor receptor (EGFR) and affect recruitment of the downstream signaling protein Grb2 [C] . Amanuel Y. Kehasse, Giuseppe Infusini, Ilene Boucher, American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2009

机译：猪主动脉内皮细胞（PAE）与核苷酸或EGF的损伤和刺激导致表皮生长因子受体（EGFR）的差异磷酸化，并影响下游信号蛋白GRB2的募集
5. An antagonist of the prostaglandin F2alpha receptor (FP)-Galphaq-dependent response, biases FP into mitogen-activated protein kinase (MAPK) signalling through epidermal growth factor receptor (EGFR) transactivation . [D] . Wisehart, Veronica. 2011

机译：前列腺素F2alpha受体（FP）-Galphaq依赖性反应的拮抗剂通过表皮生长因子受体（EGFR）反式激活将FP偏向促分裂原激活的蛋白激酶（MAPK）信号传导。
6. Epidermal growth factor (EGF) stimulates association and kinase activity of Raf-1 with the EGF receptor. [O] . H App, R Hazan, A Zilberstein, 1991

机译：表皮生长因子（EGF）刺激Raf-1与EGF受体的缔合和激酶活性。
7. Epidermal growth factor (EGF) stimulates association and kinase activity of Raf-1 with the EGF receptor. [O] . App, H, Hazan, R, Zilberstein, A, 1991

机译：表皮生长因子（EGF）刺激Raf-1与EGF受体的缔合和激酶活性。
8. Proline is Required for the Stimulation of DNA Synthesis in Hepatocyte Cultures by EGF (Epidermal Growth Factor) [R] . Houck, K. A. , Michalopoulos, G. 1985

机译：EGF（表皮生长因子）刺激肝细胞培养DNa合成需要脯氨酸

Epidermal growth factor (EGF) stimulates association and kinase activity of Raf-1 with the EGF receptor.

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