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首页> 外文期刊>Molecular and Cellular Biology >Mutations in the yeast RNA14 and RNA15 genes result in an abnormal mRNA decay rate; sequence analysis reveals an RNA-binding domain in the RNA15 protein.
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Mutations in the yeast RNA14 and RNA15 genes result in an abnormal mRNA decay rate; sequence analysis reveals an RNA-binding domain in the RNA15 protein.

机译：酵母RNA14和RNA15基因的突变导致异常的mRNA衰减率;序列分析揭示了RNA15蛋白中的RNA结合结构域。

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In Saccharomyces cerevisiae, temperature-sensitive mutations in the genes RNA14 and RNA15 correlate with a reduction of mRNA stability and poly(A) tail length. Although mRNA transcription is not abolished in these mutants, the transcripts are rapidly deadenylated as in a strain carrying an RNA polymerase B(II) temperature-sensitive mutation. This suggests that the primary defect could be in the control of the poly(A) status of the mRNAs and that the fast decay rate may be due to the loss of this control. By complementation of their temperature-sensitive phenotype, we have cloned the wild-type genes. They are essential for cell viability and are unique in the haploid genome. The RNA14 gene, located on chromosome H, is transcribed as three mRNAs, one major and two minor, which are 2.2, 1.5, and 1.1 kb in length. The RNA15 gene gives rise to a single 1.2-kb transcript and maps to chromosome XVI. Sequence analysis indicates that RNA14 encodes a 636-amino-acid protein with a calculated molecular weight of 75,295. No homology was found between RNA14 and RNA15 or between RNA14 and other proteins contained in data banks. The RNA15 DNA sequence predicts a protein of 296 amino acids with a molecular weight of 32,770. Sequence comparison reveals an N-terminal putative RNA-binding domain in the RNA15-encoded protein, followed by a glutamine and asparagine stretch similar to the opa sequences. Both RNA14 and RNA15 wild-type genes, when cloned on a multicopy plasmid, are able to suppress the temperature-sensitive phenotype of strains bearing either the rna14 or the rna15 mutation, suggesting that the encoded proteins could interact with each other.
机译：在酿酒酵母中，基因RNA14和RNA15中的温度敏感突变与mRNA稳定性和poly（A）尾巴长度的减少有关。尽管在这些突变体中没有废除mRNA转录，但转录本会像携带RNA聚合酶B（II）温度敏感突变的菌株一样迅速被去腺基化。这表明主要缺陷可能在于mRNA的poly（A）状态的控制，而快速衰减率可能是由于这种控制的丧失所致。通过互补它们的温度敏感性表型，我们克隆了野生型基因。它们对于细胞活力至关重要，并且在单倍体基因组中是独特的。位于H染色体上的RNA14基因被转录为三个mRNA，一个主要和两个次要，长度分别为2.2、1.5和1.1 kb。 RNA15基因产生一个1.2kb的转录本，并定位到XVI染色体。序列分析表明RNA14编码一种636个氨基酸的蛋白质，计算的分子量为75,295。在RNA14和RNA15之间或在RNA14和数据库中包含的其他蛋白质之间未发现同源性。 RNA15 DNA序列可预测296个氨基酸的蛋白质，分子量为32,770。序列比较揭示了RNA15编码蛋白中的N端假定的RNA结合结构域，其后是类似于opa序列的谷氨酰胺和天冬酰胺延伸序列。当将RNA14和RNA15野生型基因克隆到多拷贝质粒上时，它们能够抑制带有rna14或rna15突变的菌株的温度敏感表型，这表明编码的蛋白质可以彼此相互作用。

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