Synthetic lethal mutations suggest interactions between U5 small nuclear RNA and four proteins required for the second step of splicing.

D Frank; B Patterson; C Guthrie

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Synthetic lethal mutations suggest interactions between U5 small nuclear RNA and four proteins required for the second step of splicing.

机译：合成致死突变表明U5小核RNA与剪接第二步所需的四种蛋白质之间存在相互作用。

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To investigate the function of the U5 small nuclear ribonucleoprotein (snRNP) in pre-mRNA splicing, we have screened for factors that genetically interact with Saccharomyces cerevisiae U5 snRNA. We isolated trans-acting mutations that exacerbate the phenotypes of conditional alleles of the U5 snRNA and named these genes SLU, for synergistically lethal with U5 snRNA. SLU1 and SLU2 are essential for the first catalytic step of splicing, while SLU7 and SLU4 (an allele of PRP17 [U. Vijayraghavan, M. Company, and J. Abelson, Genes Dev. 3:1206-1216, 1989]) are required only for the second step of splicing. Furthermore, slu4-1 and slu7-1 are lethal in combination with mutations in PRP16 and PRP18, which also function in the second step, but not with mutations in factors required for the first catalytic step, such as PRP8 and PRP4. We infer from these data that SLU4, SLU7, PRP18, PRP16, and the U5 snRNA interact functionally and that a major role of the U5 snRNP is to coordinate a set of factors that are required for the completion of the second catalytic step of splicing.

机译：为了研究U5小核糖核蛋白（snRNP）在前mRNA剪接中的功能，我们筛选了与酿酒酵母U5 snRNA基因相互作用的因子。我们分离了加剧U5 snRNA条件等位基因表型的反式突变，并将这些基因命名为SLU，以与U5 snRNA协同致死。 SLU1和SLU2对于剪接的第一步是必不可少的，而SLU7和SLU4（PRP17的等位基因[U. Vijayraghavan，M. Company，and J. Abelson，Genes Dev。3：1206-1216，1989]）是必需的。仅用于拼接的第二步。此外，slu4-1和slu7-1与在第二步中也起作用的PRP16和PRP18中的突变结合在一起具有致死性，但与第一步催化步骤所需的因子（如PRP8和PRP4）中的突变没有结合。我们从这些数据推断出SLU4，SLU7，PRP18，PRP16和U5 snRNA在功能上相互作用，并且U5 snRNP的主要作用是协调完成拼接的第二催化步骤所需的一组因子。

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《Molecular and Cellular Biology》 |1992年第11期|共9页
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D Frank; B Patterson; C Guthrie;
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1. Synthetic Lethality of Yeast slt Mutations with U2 Small Nuclear RNA Mutations Suggests Functional Interactions between U2 and U5 snRNPs That Are Important for Both Steps of Pre-mRNA Splicing [J] . Deming Xu, Deborah J. Field, Shou-Jiang Tang, Molecular and Cellular Biology . 1998,第4期

机译：酵母菌slt突变与U2小核RNA突变的合成致死性表明U2和U5 snRNP之间的功能相互作用对于前mRNA剪接的两个步骤都很重要
2. The Prp18 protein stabilizes the interaction of both exons with the U5 snRNA during the second step of pre-mRNA splicing. [J] . Crotti LB, Bacikova D, Horowitz DS Genes and Development: a Journal Devoted to the Molecular Analysis of Gene Expression in Eukaryotes, Prokaryotes, and Viruses . 2007,第10期

机译：在mRNA前剪接的第二步中，Prp18蛋白稳定了两个外显子与U5 snRNA的相互作用。
3. Functional interactions between Prp8, Prp18, Slu7, and U5 snRNA during the second step of pre-mRNA splicing. [J] . Aronova A, Bacikova D, Crotti LB, RNA . 2007,第9期

机译：在mRNA前剪接的第二步期间，Prp8，Prp18，Slu7和U5 snRNA之间的功能相互作用。
4. Predicting Yeast Synthetic Lethal Genetic Interactions Using Protein Domains [C] . Li Bo, Luo Feng Bioinformatics and Biomedicine, 2009. BIBM '09 . 2009

机译：使用蛋白质结构域预测酵母合成致死的遗传相互作用
5. C-terminal flap endonuclease (rad27) mutations: Lethal interactions with a DNA ligase I mutation (cdc9-p) and suppression by proliferating cell nuclear antigen (POL30) in Saccharomyces cerevisiae. [D] . Karanja, Kenneth Kimani. 2009

机译：C末端皮瓣内切核酸酶（rad27）突变：与酿酒酵母中DNA连接酶I突变（cdc9-p）的致命相互作用以及通过增殖细胞核抗原（POL30）的抑制作用。
6. Synthetic lethal mutations suggest interactions between U5 small nuclear RNA and four proteins required for the second step of splicing. [O] . D Frank, B Patterson, C Guthrie 1992

机译：合成致死突变表明U5小核RNA与第二步剪接所需的四种蛋白质之间存在相互作用。
7. Synthetic lethal mutations suggest interactions between U5 small nuclear RNA and four proteins required for the second step of splicing. [O] . Frank, D, Patterson, B, Guthrie, C 1992

机译：合成致死突变表明U5小核RNA与第二步剪接所需的四种蛋白质之间存在相互作用。

Synthetic lethal mutations suggest interactions between U5 small nuclear RNA and four proteins required for the second step of splicing.

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