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首页> 外文期刊>Molecular and Cellular Biology >Chromosomal organization of Xenopus laevis oocyte and somatic 5S rRNA genes in vivo.
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Chromosomal organization of Xenopus laevis oocyte and somatic 5S rRNA genes in vivo.

机译：非洲爪蟾卵母细胞和体细胞5S rRNA基因的染色体组织体内。

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We describe the chromosomal organization of the major oocyte and somatic 5S RNA genes of Xenopus laevis in chromatin isolated from erythrocyte nuclei. Both major oocyte and somatic 5S DNA repeats are associated with nucleosomes; however, differences exist in the organization of chromatin over the oocyte and somatic 5S RNA genes. The repressed oocyte 5S RNA gene is protected from nuclease digestion by incorporation into a nucleosome, and the entire oocyte 5S DNA repeat is assembled into a loosely positioned array of nucleosomes. In contrast, the potentially active somatic 5S RNA gene is accessible to nuclease digestion, and the majority of somatic 5S RNA genes appear not to be incorporated into positioned nucleosomes. Evidence is presented supporting the stable association of transcription factors with the somatic 5S RNA genes. Histone H1 is shown to have a role both in determining the organization of nucleosomes over the oocyte 5S DNA repeat and in repressing transcription of the oocyte 5S RNA genes.
机译：我们描述了从红血球核分离的染色质中非洲爪蟾的主要卵母细胞和体细胞5S RNA基因的染色体组织。主要的卵母细胞和体细胞5S DNA重复序列均与核小体相关。然而，卵母细胞和体细胞5S RNA基因的染色质组织存在差异。通过整合到核小体中，可以保护被抑制的卵母细胞5S RNA基因免受核酸酶消化，并且将整个卵母5S DNA重复序列组装成一个松散排列的核小体阵列。相反，潜在活性的体细胞5S RNA基因可被核酸酶消化，并且大多数体细胞5S RNA基因似乎没有被掺入定位的核小体中。提出的证据支持转录因子与体细胞5S RNA基因的稳定关联。显示组蛋白H1在确定卵母细胞5S DNA重复序列上的核小体的组织以及抑制卵母细胞5S RNA基因的转录中均具有作用。

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