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首页> 外文期刊>Molecular and Cellular Biology >Identification of a new set of cell cycle-regulatory genes that regulate S-phase transcription of histone genes in Saccharomyces cerevisiae.
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Identification of a new set of cell cycle-regulatory genes that regulate S-phase transcription of histone genes in Saccharomyces cerevisiae.

机译：鉴定一组新的细胞周期调控基因，这些基因调控酿酒酵母中组蛋白基因的S期转录。

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Histone mRNA synthesis is tightly regulated to S phase of the yeast Saccharomyces cerevisiae cell cycle as a result of transcriptional and posttranscriptional controls. Moreover, histone gene transcription decreases rapidly if DNA replication is inhibited by hydroxyurea or if cells are arrested in G1 by the mating pheromone alpha-factor. To identify the transcriptional controls responsible for cycle-specific histone mRNA synthesis, we have developed a selection for mutations which disrupt this process. Using this approach, we have isolated five mutants (hpc1, hpc2, hpc3, hpc4, and hpc5) in which cell cycle regulation of histone gene transcription is altered. All of these mutations are recessive and belong to separate complementation groups. Of these, only one (hpc1) falls in one of the three complementation groups identified previously by other means (M. A. Osley and D. Lycan, Mol. Cell. Biol. 7:4204-4210, 1987), indicating that at least seven different genes are involved in the cell cycle-specific regulation of histone gene transcription. hpc4 is unique in that derepression occurs only in the presence of hydroxyurea but not alpha-factor, suggesting that at least one of the regulatory factors is specific to histone gene transcription after DNA replication is blocked. One of the hpc mutations (hpc2) suppresses delta insertion mutations in the HIS4 and LYS2 loci. This effect allowed the cloning and sequence analysis of HPC2, which encodes a 67.5-kDa, highly charged basic protein.
机译：由于转录和转录后控制，组蛋白mRNA合成被严格调控到酿酒酵母细胞周期的S期。此外，如果DNA复制受到羟基脲的抑制或如果细胞被交配的信息素α因子阻滞在G1中，则组蛋白基因转录会迅速降低。为了鉴定负责周期特异性组蛋白mRNA合成的转录控制，我们已经选择了破坏该过程的突变。使用这种方法，我们分离了五个突变体（hpc1，hpc2，hpc3，hpc4和hpc5），其中组蛋白基因转录的细胞周期调控发生了改变。所有这些突变都是隐性的，属于单独的互补组。其中，只有一个（hpc1）属于先前通过其他方式鉴定的三个互补组之一（MA Osley和D. Lycan，Mol。Cell。Biol。7：4204-4210，1987），表明至少有七个不同的基因参与组蛋白基因转录的细胞周期特异性调控。 hpc4的独特之处在于，仅在羟基脲的存在下才发生抑制，而不在α因子的存在下发生，这表明在DNA复制受阻后，至少一种调控因子对组蛋白基因转录具有特异性。 hpc突变之一（hpc2）抑制HIS4和LYS2基因座中的delta插入突变。这种作用允许克隆和编码67.5 kDa，高度带电的碱性蛋白的HPC2进行序列分析。

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