• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular and Cellular Biology >Expression of p60v-src in Saccharomyces cerevisiae results in elevation of p34CDC28 kinase activity and release of the dependence of DNA replication on mitosis.
【24h】

Expression of p60v-src in Saccharomyces cerevisiae results in elevation of p34CDC28 kinase activity and release of the dependence of DNA replication on mitosis.

机译：p60v-src在酿酒酵母中的表达导致p34CDC28激酶活性升高，并释放DNA复制对有丝分裂的依赖性。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Expression of the oncogenic protein tyrosine kinase p60v-src in the yeast Saccharomyces cerevisiae has been shown to result in rapid cell death (J. S. Brugge, G. Jarosik, J. Andersen, A. Queral-Lustig, M. Fedor-Chaiken, and J. R. Broach, Mol. Cell. Biol. 7:2180-2187, 1987). Work described here demonstrates that v-Src expression results in accumulation of large-budded cells and a nuclear division block without blocking cytokinesis. Flow-cytometric analysis indicates that the DNA content of these cells is elevated beyond the G2 DNA content, and genetic studies indicate that v-Src expression causes aneuploidy. The activity of Cdc28 kinase, which controls the G1/S and G2/M transitions in S. cerevisiae, increases during galactose induction in a Src+ strain but not in an isogenic Src- strain. These observations indicate that v-Src expression disrupts p34CDC28 kinase regulation, allowing DNA replication to proceed in the absence of a prior mitotic event.
机译：致癌蛋白酪氨酸激酶p60v-src在酿酒酵母中的表达已显示可导致细胞快速死亡（JS Brugge，G。Jarosik，J。Andersen，A。Queral-Lustig，M。Fedor-Chaiken和JR Broach，Mol.Cell.Biol.7：2180-2187，1987）。此处描述的工作证明v-Src表达导致大预算细胞的积累和核分裂阻滞，而不会阻止胞质分裂。流式细胞仪分析表明这些细胞的DNA含量增加到超过G2 DNA含量，遗传研究表明v-Src表达引起非整倍性。控制S.cerevisiae中G1 / S和G2 / M转变的Cdc28激酶的活性在半乳糖诱导过程中在Src +菌株中增加，但在同基因Src-菌株中不增加。这些观察结果表明，v-Src表达破坏了p34CDC28激酶的调控，从而使DNA复制在没有先前有丝分裂事件的情况下得以进行。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号