Transcriptional up-regulation of the mouse cytosolic glutathione peroxidase gene in erythroid cells is due to a tissue-specific 3' enhancer containing functionally important CACC/GT motifs and binding sites for GATA and Ets transcription factors.

J OPrey; S Ramsay; I Chambers; P R Harrison

首页> 外文期刊>Molecular and Cellular Biology >Transcriptional up-regulation of the mouse cytosolic glutathione peroxidase gene in erythroid cells is due to a tissue-specific 3' enhancer containing functionally important CACC/GT motifs and binding sites for GATA and Ets transcription factors.

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Transcriptional up-regulation of the mouse cytosolic glutathione peroxidase gene in erythroid cells is due to a tissue-specific 3' enhancer containing functionally important CACC/GT motifs and binding sites for GATA and Ets transcription factors.

机译：红细胞中小鼠胞质谷胱甘肽过氧化物酶基因的转录上调归因于组织特异性3'增强子，其中包含功能上重要的CACC / GT基序以及GATA和Ets转录因子的结合位点。

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Nuclear run-on experiments have shown that the high level of expression of the mouse cytosolic glutathione peroxidase mRNA in erythroid cells is due to up-regulation of the gene at the transcriptional level. Studies of the chromatin structure around the cytosolic glutathione peroxidase gene have revealed a series of DNase I hypersensitive sites (DHSS) in the 3' flanking region of the gene in erythroid and other high-expression tissues that are lacking in low-expression cells, in addition to a DHSS over the promoter region in both high- and low-expression tissues. Functional transfection experiments have demonstrated that one of the 3' DHSS regions functions as an enhancer in erythroid cells but not in a low-expression epithelial cell line; and site-directed mutagenesis and footprinting experiments reveal that the activity of the erythroid cell-specific enhancer requires a cluster of binding sites for the CACC/GT box factors and the GATA and Ets families of transcription factors.

机译：核实验表明，红细胞中小鼠胞质谷胱甘肽过氧化物酶mRNA的高水平表达是由于该基因在转录水平上的上调。对胞质谷胱甘肽过氧化物酶基因周围染色质结构的研究表明，在红系和其他低表达细胞缺乏的高表达组织中，该基因的3'侧翼区域存在一系列DNase I超敏位点（DHSS）。在高表达和低表达组织中，除了在启动子区域上的DHSS外。功能性转染实验已证明3'DHSS区域之一在红系细胞中起增强子的作用，而在低表达上皮细胞系中不起作用。定点诱变和足迹实验表明，类红细胞特异性增强子的活性需要CACC / GT盒因子以及GATA和Ets转录因子家族的结合位点簇。

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《Molecular and Cellular Biology》 |1993年第10期|共14页
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J OPrey; S Ramsay; I Chambers; P R Harrison;
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1. Transcriptional activation of human adult α-globin genes by hypersensitive site-40 enhancer: Function of nuclear factor-binding motifs occupied in erythroid cells [J] . IRENE ROMBEL, KUANG-YU HU, QINGYI ZHANG, Proceedings of the National Academy of Sciences of the United States of America . 1995,第14期

机译：过敏性位点40增强子对人类成人α-珠蛋白基因的转录激活：红细胞中占据的核因子结合基序的功能
2. An endoderm-specific transcriptional enhancer from the mouse Gata4 gene requires GATA and homeodomain protein-binding sites for function in vivo. [J] . Rojas A, Schachterle W, Xu SM, Developmental dynamics: an official publication of the American Association of Anatomists . 2009,第10期

机译：小鼠Gata4基因的内胚层特异性转录增强子需要GATA和同源结构域蛋白结合位点才能发挥体内功能。
3. An endoderm-specific transcriptional enhancer from the mouse Gata4 gene requires GATA and homeodomain protein-binding sites for function in vivo. [J] . Rojas A, Schachterle W, Xu SM, Developmental dynamics: an official publication of the American Association of Anatomists . 2009,第10期

机译：来自小鼠GATA4基因的内胚层特异性转录增强剂需要GATA和同源域的蛋白质结合位点，用于体内功能。
4. Assessing phylogenetic motif models for predicting transcription factor binding sites [C] . John Hawkins, Charles Grant, William Stafford Noble, Intelligent Systems for Molecular Biology . 2009

机译：评估用于预测转录因子结合位点的系统发育基序模型
5. Identifying Binding Sites, Target Genes, and Functional Domains of the T-box Transcription Factor midline. [D] . Najand, Nima. 2014

机译：识别T盒转录因子中线的结合位点，靶基因和功能域。
6. Transcriptional up-regulation of the mouse cytosolic glutathione peroxidase gene in erythroid cells is due to a tissue-specific 3 enhancer containing functionally important CACC/GT motifs and binding sites for GATA and Ets transcription factors. [O] . J OPrey, S Ramsay, I Chambers, 1994

机译：红细胞中小鼠胞质谷胱甘肽过氧化物酶基因的转录上调归因于组织特异性3增强子其中包含功能上重要的CACC / GT基序以及GATA和Ets转录因子的结合位点。
7. Transcriptional up-regulation of the mouse cytosolic glutathione peroxidase gene in erythroid cells is due to a tissue-specific 3' enhancer containing functionally important CACC/GT motifs and binding sites for GATA and Ets transcription factors. [O] . O'Prey, J, Ramsay, S, Chambers, I, 1994

机译：红细胞中小鼠胞质谷胱甘肽过氧化物酶基因的转录上调归因于组织特异性3'增强子，其中包含功能上重要的CACC / GT基序以及GATA和Ets转录因子的结合位点。

Transcriptional up-regulation of the mouse cytosolic glutathione peroxidase gene in erythroid cells is due to a tissue-specific 3' enhancer containing functionally important CACC/GT motifs and binding sites for GATA and Ets transcription factors.

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