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首页> 外文期刊>Molecular and Cellular Biology >A phylogenetically conserved sequence within viral 3' untranslated RNA pseudoknots regulates translation.
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A phylogenetically conserved sequence within viral 3' untranslated RNA pseudoknots regulates translation.

机译：病毒3'非翻译RNA假结内的系统发育保守序列调节翻译。

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摘要

Both the 68-base 5' leader (omega) and the 205-base 3' untranslated region (UTR) of tobacco mosaic virus (TMV) promote efficient translation. A 35-base region within omega is necessary and sufficient for the regulation. Within the 3' UTR, a 52-base region, composed of two RNA pseudoknots, is required for regulation. These pseudoknots are phylogenetically conserved among seven viruses from two different viral groups and one satellite virus. The pseudoknots contained significant conservation at the secondary and tertiary levels and at several positions at the primary sequence level. Mutational analysis of the sequences determined that the primary sequence in several conserved positions, particularly within the third pseudoknot, was essential for function. The higher-order structure of the pseudoknots was also required. Both the leader and the pseudoknot region were specifically recognized by, and competed for, the same proteins in extracts made from carrot cell suspension cells and wheat germ. Binding of the proteins is much stronger to omega than the pseudoknot region. Synergism was observed between the TMV 3' UTR and the cap and to a lesser extent between omega and the 3' UTR. The functional synergism and the protein binding data suggest that the cap, TMV 5' leader, and 3' UTR interact to establish an efficient level of translation.
机译：烟草花叶病毒（TMV）的68个碱基的5'前导序列（omega）和205个碱基的3'非翻译区（UTR）均可促进有效翻译。欧米茄内35个碱基的区域对于调节而言是必要且足够的。在3'UTR内，需要由两个RNA假结组成的52个碱基的区域进行调控。这些假结在两个不同病毒组的七个病毒和一个卫星病毒之间在系统发育上是保守的。假结在二级和三级以及一级序列的几个位置上均具有明显的保守性。序列的突变分析确定，在几个保守位置，特别是在第三个假结内，主要序列对功能至关重要。还需要伪结的高阶结构。前导区和假结区都被胡萝卜细胞悬浮细胞和小麦胚芽制成的提取物中的相同蛋白质特异性识别并竞争。与假结区域相比，蛋白质与ω的结合要强得多。在TMV 3'UTR和瓶盖之间观察到协同作用，在欧米茄和3'UTR之间观察到较小程度的协同作用。功能协同作用和蛋白质结合数据表明，帽，TMV 5'前导序列和3'UTR相互作用以建立有效的翻译水平。

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