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首页> 外文期刊>Molecular and Cellular Biology >A role for RNA synthesis in homologous pairing events.
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A role for RNA synthesis in homologous pairing events.

机译：RNA合成在同源配对事件中的作用。

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The relationship between RNA synthesis and homologous pairing in vitro, catalyzed by RecA protein, was examined by using an established strand transfer assay system. When a short DNA duplex is mixed with single-stranded circles, RecA protein promotes the transfer of the minus strand of the duplex onto the complementary region of the plus-strand circle, with the displacement of the plus strand of the duplex. However, if minus-strand RNA is synthesized from the duplex pairing partner, joint molecules containing the RNA transcript, the plus strand of the DNA duplex, and the plus-strand circle are also observed to form. This reaction, which is dependent on RNA polymerase, sequence homology, and RecA protein, produces a joint molecule that can be dissolved by treatment with RNase H but not RNase A. Under these reaction conditions, product molecules form even when the length of shared homology between duplex and circle is reduced to 15 bp.
机译：通过使用已建立的链转移测定系统，研究了RecA蛋白催化的RNA合成与体外同源配对之间的关​​系。当短的DNA双链体与单链环混合时，RecA蛋白会促进双链体的负链转移到正链环的互补区域上，并置换双链体的正链。但是，如果从双链体配对伴侣合成了负链RNA，也会观察到含有RNA转录物，DNA双链体的正链和正链环的联合分子。该反应取决于RNA聚合酶，序列同源性和RecA蛋白，产生的联合分子可以通过RNase H而不是RNase A的处理而溶解。在这些反应条件下，即使共有同源长度，产物分子也会形成双链体和环之间的距离减少到15 bp。

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