...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular and Cellular Biology >Effects of c-myc expression on cell cycle progression.
【24h】

Effects of c-myc expression on cell cycle progression.

机译：c-myc表达对细胞周期进程的影响。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We used targeted homologous recombination to disrupt one c-myc gene copy in a diploid fibroblast cell line and found that a twofold reduction in Myc expression resulted in lower exponential growth rates and a lengthening of the G0-to-S-phase transition (M. Shichiri, K. D. Hanson and J. M. Sedivy, Cell Growth Differ. 4:93-104, 1993). Myc is a transcription factor, and the number of target genes whose regulation could result in differential growth rates may be very large. We have approached this problem by examining effects of reduced c-myc expression in three broad areas: (i) secretion of growth factors, (ii) expression of growth factor receptors, and (iii) intracellular signal transduction between Myc and components of the intrinsic cell cycle clock. We have found no evidence that differential medium conditioning can account for the growth phenotypes. Likewise, the expression of receptors for platelet-derived growth factor, epidermal growth factor, basic fibroblast growth factor, and insulin-like growth factor I was the same in diploid and heterozygous cells (platelet-derived growth factor, epidermal growth factor, fibroblast growth factor, and insulin-like growth factor are the sole growth factors required by these cells for growth in serum-free medium). In contrast, expression of cyclin E, cyclin A, and Rb phosphorylation were delayed when quiescent c-myc heterozygous cells were stimulated to enter the cell cycle. Expression of cyclin D1, cyclin D3, and Cdk2 was not affected. The timing of cyclin E induction was the earliest observable effect of reduced Myc expression. Our data indicate that Myc contributes to regulation of proliferation by a cell-autonomous mechanism that involves the modulation of cyclin E expression and, consequently, progression through the restriction point of the cell cycle.
机译：我们使用靶向同源重组破坏了二倍体成纤维细胞系中的一个c-myc基因拷贝，发现Myc表达的两倍降低导致指数增长速率降低和G0到S相转变的延长（M. Shichiri，KD Hanson和JM Sedivy，Cell Growth Differ.4：93-104，1993）。 Myc是转录因子，其调控可能导致不同的生长速率的靶基因数量可能非常多。我们通过研究降低的c-myc表达在三个主要领域的影响来解决这个问题：（i）生长因子的分泌，（ii）生长因子受体的表达，以及（iii）Myc与内在成分之间的细胞内信号转导细胞周期时钟。我们没有发现差异培养基条件可以解释生长表型的证据。同样，在二倍体和杂合细胞中，血小板衍生生长因子，表皮生长因子，碱性成纤维细胞生长因子和胰岛素样生长因子I的受体表达相同（血小板衍生生长因子，表皮生长因子，成纤维细胞生长这些因子在无血清培养基中生长所需的唯一生长因子是胰岛素样生长因子和胰岛素样生长因子。相反，当刺激静止的c-myc杂合细胞进入细胞周期时，细胞周期蛋白E，细胞周期蛋白A和Rb磷酸化的表达被延迟。细胞周期蛋白D1，细胞周期蛋白D3和Cdk2的表达不受影响。细胞周期蛋白E诱导的时间是减少Myc表达的最早可观察到的作用。我们的数据表明，Myc通过涉及细胞周期蛋白E表达的调节的细胞自主机制来促进增殖的调节，因此，它通过细胞周期的限制点而发展。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号