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首页> 外文期刊>Molecular and Cellular Biology >Association of p107 with Sp1: genetically separable regions of p107 are involved in regulation of E2F- and Sp1-dependent transcription.
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Association of p107 with Sp1: genetically separable regions of p107 are involved in regulation of E2F- and Sp1-dependent transcription.

机译：p107与Sp1的关联：p107的基因可分离区域参与E2F和Sp1依赖性转录的调节。

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摘要

The retinoblastoma-related protein p107 has been shown to be a regulator of the transcription factor E2F. p107 associates with E2F via its pocket region and represses E2F-dependent transcription. In this study, we provide evidence for a novel interaction between p107 and the transcription factor Sp1. We show that p107 can be found endogenously associated with Sp1 in the extracts of several different cell lines. Moreover, in transient transfection assays, expression of p107 represses Sp1-dependent transcription. This repression of Sp1-dependent transcription does not require the DNA-binding domain of Sp1. Transcription driven by a chimeric protein containing the Ga14 DNA-binding domain and the Sp1 activation domains is inhibited by p107. Interestingly, unlike the repression of E2F-dependent transcription, the repression of Sp1-dependent transcription does not depend on an intact pocket region. We show that distinct regions of p107 are involved in the control of Sp1 and E2F.
机译：视网膜母细胞瘤相关蛋白p107已被证明是转录因子E2F的调节剂。 p107通过其口袋区域与E2F结合并抑制E2F依赖性转录。在这项研究中，我们为p107和转录因子Sp1之间的新型相互作用提供了证据。我们表明，p107可以在几种不同细胞系的提取物中与Sp1内源性结合。此外，在瞬时转染测定中，p107的表达抑制Sp1依赖性转录。 Sp1依赖转录的这种压制不需要Sp1的DNA结合域。 p107抑制了由含有Ga14 DNA结合结构域和Sp1激活结构域的嵌合蛋白驱动的转录。有趣的是，与E2F依赖性转录的抑制不同，Sp1依赖性转录的抑制不依赖完整的口袋区域。我们显示p107的不同区域参与Sp1和E2F的控制。

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