• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular and Cellular Biology >Multiple molecular determinants for retrotransposition in a primer tRNA.
【24h】

Multiple molecular determinants for retrotransposition in a primer tRNA.

机译：引物tRNA中逆转录的多个分子决定簇。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Retroviruses and long terminal repeat-containing retroelements use host-encoded tRNAs as primers for the synthesis of minus strong-stop DNA, the first intermediate in reverse transcription of the retroelement RNA. Usually, one or more specific tRNAs, including the primer, are selected and packaged within the virion. The reverse transcriptase (RT) interacts with the primer tRNA and initiates DNA synthesis. The structural and sequence features of primer tRNAs important for these specific interactions are poorly understood. We have developed a genetic assay in which mutants of tRNA(iMet), the primer for the Ty1 retrotransposon of Saccharomyces cerevisiae, can be tested for the ability to serve as primers in the reverse transcription process. This system allows any tRNA mutant to be tested, regardless of its ability to function in the initiation of protein synthesis. We find that mutations in the T psi C loop and the acceptor stem regions of the tRNA(iMet) affect transposition most severely. Conversely, mutations in the anticodon region have only minimal effects on transposition. Further study of the acceptor stem and other mutants demonstrates that complementarity to the element primer binding site is a necessary but not sufficient requirement for effective tRNA priming. Finally, we have used interspecies hybrid initiator tRNA molecules to implicate nucleotides in the D arm as additional recognition determinants. Ty3 and Ty1, two very distantly related retrotransposons, require similar molecular determinants in this primer tRNA for transposition.
机译：逆转录病毒和含有长末端重复序列的逆转录元件使用宿主编码的tRNA作为合成负强终止DNA的引物，DNA是逆转录元件RNA逆转录的第一个中间产物。通常，选择一种或多种特异性tRNA，包括引物，并将其包装在病毒体中。逆转录酶（RT）与引物tRNA相互作用并启动DNA合成。对于这些特异性相互作用重要的引物tRNA的结构和序列特征了解甚少。我们已经开发了一种遗传检测方法，其中可以测试tRNA（iMet）突变体（酿酒酵母Ty1反转录转座子的引物）在逆转录过程中用作引物的能力。该系统允许测试任何tRNA突变体，无论其在蛋白质合成启动中发挥功能的能力如何。我们发现，T psi C环和tRNA（iMet）的受体茎区域中的突变对转座的影响最大。相反，反密码子区域的突变对转座的影响很小。受体茎和其他突变体的进一步研究表明，与元件引物结合位点的互补性对于有效的tRNA引发是必要但不是充分的要求。最后，我们使用种间杂种起始子tRNA分子将D臂中的核苷酸暗示为其他识别决定因素。 Ty3和Ty1，两个非常相关的逆转座子，在此引物tRNA中需要相似的分子决定子来进行转座。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号