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首页> 外文期刊>Molecular and Cellular Biology >The sak1+ gene of Schizosaccharomyces pombe encodes an RFX family DNA-binding protein that positively regulates cyclic AMP-dependent protein kinase-mediated exit from the mitotic cell cycle.
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The sak1+ gene of Schizosaccharomyces pombe encodes an RFX family DNA-binding protein that positively regulates cyclic AMP-dependent protein kinase-mediated exit from the mitotic cell cycle.

机译：粟酒裂殖酵母的sak1 +基因编码一个RFX家族DNA结合蛋白，可正向调节环状AMP依赖性蛋白激酶介导的有丝分裂细胞周期的退出。

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In Schizosaccharomyces pombe, meiosis is initiated by conditions of nutrient deprivation. Mutations in genes encoding elements of the cyclic AMP-dependent protein kinase (cAPK) pathway interfere with meiosis. Loss-of-function alleles of genes that stimulate the activity of cAPK allow cells to bypass the normal requirement of starvation for conjugation and meiosis. Alternatively, loss-of-function alleles of genes that inhibit cAPK lead to the inability to undergo sexual differentiation. The cgs1+ gene encodes the regulatory subunit of cAPK, and the cgs2+ gene encodes a cyclic AMP phosphodiesterase. Thus, both genes encode proteins which negatively regulate the activity of cAPK. Loss of either cgs1 or cgs2 prevents haploid cells from conjugating and diploid cells from undergoing meiosis. In addition to these defects, cells are unable to enter stationary phase. We describe a novel gene, sak1+, which when present on a plasmid overcomes the aberrant phenotypes associated with unregulated cAPK activity. Genetic analysis of sak1+ (suppressor of A-kinase) reveals that it functions downstream of cyclic AMP-dependent protein kinase to allow cells to exist the mitotic cycle and enter either stationary phase or the pathway leading to sexual differentiation. The sak1+ gene is essential for cell viability, and a null allele causes multiple defects in cell morphology and nuclear division. Thus, sak1+ is an important regulatory element in the life cycle of S. pombe. Sequence analysis shows that the predicted product of the sak1+ gene is an 87-kDa protein which shares homology to the RFX family of DNA-binding proteins identified in humans and mice. One member of this family, RFX1, is a transcription factor for a variety of viral and cellular genes.
机译：在粟酒裂殖酵母中，减数分裂是由营养缺乏引起的。编码环AMP依赖蛋白激酶（cAPK）途径的元件的基因中的突变会干扰减数分裂。刺激cAPK活性的基因的功能丧失等位基因使细胞绕过饥饿正常的共轭和减数分裂需求。或者，抑制cAPK的基因功能缺失的等位基因导致无法进行性别分化。 cgs1 +基因编码cAPK的调节亚基，而cgs2 +基因编码环状AMP磷酸二酯酶。因此，两个基因都编码负调节cAPK活性的蛋白质。 cgs1或cgs2的丢失会阻止单倍体细胞共轭，阻止二倍体细胞减数分裂。除这些缺陷外，细胞无法进入固定相。我们描述了一个新的基因，sak1 +，当存在于质粒上时，克服了与不受调节的cAPK活性有关的异常表型。 sak1 +（A激酶抑制剂）的遗传分析表明，它在环状AMP依赖性蛋白激酶的下游起作用，使细胞存在有丝分裂循环并进入固定相或进入导致性分化的途径。 sak1 +基因对于细胞活力至关重要，而等位基因无效会导致细胞形态和核分裂的多种缺陷。因此，sak1 +是粟酒裂殖酵母生命周期中的重要调控元素。序列分析表明，sak1 +基因的预测产物是一个87 kDa的蛋白质，与人和小鼠中发现的RFX家族的DNA结合蛋白具有同源性。该家族的一个成员RFX1是多种病毒和细胞基因的转录因子。

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