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首页> 外文期刊>Molecular and Cellular Biology >Interaction of C/EBPbeta and v-Myb is required for synergistic activation of the mim-1 gene.
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Interaction of C/EBPbeta and v-Myb is required for synergistic activation of the mim-1 gene.

机译：C / EBPbeta和v-Myb的相互作用是mim-1基因协同激活所必需的。

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摘要

The retroviral oncogene v-myb encodes a transcription factor (v-Myb) which activates the myelomonocyte-specific mim-1 gene, a natural myb target gene, by cooperating with members of the C/EBP transcription factor family. The finding that v-Myb, together with C/EBP, is sufficient to activate the mim-1 gene in heterologous cell types has implicated Myb and C/EBP as a bipartite molecular switch, which regulates the expression of myelomonocyte-specific genes. To understand the relationship between v-Myb and C/EBP in more detail, we have examined the molecular basis of the activation of the mim-1 promoter by v-Myb and C/EBPbeta, a member of the C/EBP transcription factor family highly expressed in myelomonocytic cells. We have identified a composite Myb and C/EBP response element which mediates synergistic activation of the mim-1 promoter by both factors and consists of closely spaced Myb- and C/EBP-binding sites. In vitro and in vivo protein-binding studies indicate that v-Myb and C/EBPbeta interact with each other via their DNA-binding domains. We show that this interaction is essential for the synergistic activation of the mim-1 promoter by v-Myb and C/EBPbeta. Our work therefore identifies C/EBPbeta as an interaction partner of v-Myb involved in myelomonocyte gene expression.
机译：逆转录病毒癌基因v-myb编码一个转录因子（v-Myb），该转录因子通过与C / EBP转录因子家族的成员合作，激活了骨髓细胞特异性mim-1基因（一种天然的myb靶基因）。 v-Myb与C / EBP一起足以激活异源细胞类型中的mim-1基因的发现，已暗示Myb和C / EBP是二聚体分子开关，可调节骨髓单核细胞特异性基因的表达。为了更详细地了解v-Myb和C / EBP之间的关系，我们检查了v-Myb和C / EBP转录因子家族成员C / EBPbeta激活mim-1启动子的分子基础。在骨髓单核细胞中高度表达。我们已经确定了一个复合的Myb和C / EBP响应元件，它通过两个因素介导mim-1启动子的协同激活，并由间隔紧密的Myb-和C / EBP结合位点组成。体外和体内蛋白质结合研究表明，v-Myb和C / EBPbeta通过其DNA结合结构域彼此相互作用。我们显示这种相互作用对于v-Myb和C / EBPbeta对mim-1启动子的协同激活至关重要。因此，我们的工作将C / EBPbeta确定为参与粒细胞基因表达的v-Myb的相互作用伴侣。

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