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首页> 外文期刊>Molecular and Cellular Biology >Three genes for the elongation factor EF-1 alpha in Mucor racemosus.
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Three genes for the elongation factor EF-1 alpha in Mucor racemosus.

机译：芥菜中伸长因子EF-1α的三个基因。

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摘要

We cloned three genes from Mucor racemosus coding for protein synthesis elongation factor 1 alpha (EF-1 alpha). A 110-base-pair (bp) EF-1 alpha-specific cDNA clone was identified by hybrid-selected translation. The nucleotide sequence of the cDNA showed significant homology to a region of the Saccharomyces cerevisiae genes for EF-1 alpha (TEF1 and TEF2). The cDNA was used to isolate an 850-bp EcoRI genomic DNA fragment containing a portion of the EF-1 alpha gene. Screening of a lambda/M. racemosus genomic DNA bank with the 850-bp EcoRI probe resulted in the identification of three DNA fragments containing a common 850-bp EcoRI fragment within a short overlapping region. S1 nuclease analysis of the three EF-1 alpha DNA fragments showed that the EF-1 alpha transcript covered the short overlapping region in the clones. Restriction fragments purified from flanking regions in each clone were used to probe a HindIII digest of M. racemosus genomic DNA. Each flanking probe hybridized to one of three DNA fragments which hybridized to the 850-bp EF-1 alpha-specific probe. Nucleotide sequence data from two random "shotgun clones" of one of the three genes show good homology to two regions of S. cerevisiae TEF1. The data indicate the presence of three genes for EF-1 alpha in M. racemosus located at unique sites in the genome.
机译：我们从Mucor racemosus克隆了三个编码蛋白质合成延伸因子1 alpha（EF-1 alpha）的基因。通过杂交选择翻译鉴定出110个碱基对（bp）的EF-1α-特异性cDNA克隆。 cDNA的核苷酸序列与EF-1 alpha（TEF1和TEF2）的酿酒酵母基因区域具有显着同源性。该cDNA被用于分离包含一部分EF-1α基因的850bp的EcoRI基因组DNA片段。 λ/ M的筛选。带有850-bp EcoRI探针的racemosus基因组DNA库在短重叠区域内鉴定出三个含有850-bp EcoRI片段的DNA片段。对三个EF-1 alpha DNA片段的S1核酸酶分析表明，EF-1 alpha转录本覆盖了克隆中的短重叠区。从每个克隆的侧翼区域中纯化的限制性片段被用来探测总异形支原体基因组DNA的HindIII消化物。每个侧翼探针与三个DNA片段之一杂交，该三个DNA片段与850 bp EF-1α特异性探针杂交。来自三个基因之一的两个随机“ shot弹枪克隆”的核苷酸序列数据显示与酿酒酵母TEF1的两个区域具有良好的同源性。数据表明位于基因组独特位点的总状分枝杆菌中存在EF-1α的三个基因。

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