Substrate recognition by the 2 micron circle site-specific recombinase: effect of mutations within the symmetry elements of the minimal substrate.

P V Prasad; D Horensky; L J Young; M Jayaram

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Substrate recognition by the 2 micron circle site-specific recombinase: effect of mutations within the symmetry elements of the minimal substrate.

机译：2微米圈位点特异性重组酶对底物的识别：最小底物对称元件内突变的影响。

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The minimal substrate for the 2 microns circle site-specific recombinase FLP consists of a nearly perfect 13-base-pair dyad symmetry with an 8-base-pair core. By using a series of chemically synthesized FLP substrates in in vitro FLP recombination and FLP-binding assays, we have identified four positions within each of the symmetry elements that are important contact points for the FLP protein. Furthermore, the binding and recombination data provide evidence for cooperativity between the two symmetry elements of a substrate and between the symmetry elements of two partner substrates during FLP recombination.

机译：2微米圆圈位点特异性重组酶FLP的最小底物由具有8个碱基对核心的近乎完美的13个碱基对二元组对称组成。通过在体外FLP重组和FLP结合测定中使用一系列化学合成的FLP底物，我们确定了每个对称元件中的四个位置，它们是FLP蛋白的重要接触点。此外，结合和重组数据提供了在FLP重组期间底物的两个对称元件之间以及两个伙伴底物的对称元件之间的协同作用的证据。

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《Molecular and Cellular Biology》 |1986年第12期|共6页
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P V Prasad; D Horensky; L J Young; M Jayaram;
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中图分类细胞生物学;
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1. Mutations that improve the binding of yeast FLP recombinase to its substrate. [J] . B Lebreton, M Jayaram, P V Prasad, Genetics: A Periodical Record of Investigations Bearing on Heredity and Variation . 1988,第3期

机译：改善酵母FLP重组酶与其底物结合的突变。
2. Functional analysis of box I mutations in yeast site-specific recombinases Flp and R: pairwise complementation with recombinase variants lacking the active-site tyrosine. [J] . J W Chen, B R Evans, S H Yang, Molecular and Cellular Biology . 1992,第9期

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3. Analysis of suppressor mutations of spoIVCA mutations: occurrence of DNA rearrangement in the absence of site-specific DNA recombinase SpoIVCA in Bacillus subtilis. [J] . T Sato, K Harada, Y Kobayashi Journal of bacteriology . 1996,第11期

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4. Noninertial symmetry group with invariant Minkowski line element consistent with Heisenberg quantum commutation relations [C] . Stephen G. Low QTS6 . 2009

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5. Laser assisted removal of micron and sub micron particulate contamination from semiconductor and optically transparent substrate. [D] . Shukla, Shishir Dinanath. 2007

机译：激光辅助去除半导体和光学透明基板上的微米和亚微米颗粒污染物。
6. Substrate recognition by the 2 micron circle site-specific recombinase: effect of mutations within the symmetry elements of the minimal substrate. [O] . P V Prasad, D Horensky, L J Young, 1986

机译：2微米圈位点特异性重组酶对底物的识别：最小底物对称元件内突变的影响。
7. Substrate recognition by the 2 micron circle site-specific recombinase: effect of mutations within the symmetry elements of the minimal substrate. [O] . Prasad, P V, Horensky, D, Young, L J, 1986

机译：2微米圈位点特异性重组酶对底物的识别：最小底物对称元件内突变的影响。

Substrate recognition by the 2 micron circle site-specific recombinase: effect of mutations within the symmetry elements of the minimal substrate.

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