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首页> 外文期刊>Molecular and Cellular Biology >Molecular analysis of SNF2 and SNF5, genes required for expression of glucose-repressible genes in Saccharomyces cerevisiae.
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Molecular analysis of SNF2 and SNF5, genes required for expression of glucose-repressible genes in Saccharomyces cerevisiae.

机译：SNF2和SNF5（在酿酒酵母中表达葡萄糖可抑制基因所需的基因）的分子分析。

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摘要

The SNF2 and SNF5 genes are required for derepression of SUC2 and other glucose-repressible genes of Saccharomyces cerevisiae in response to glucose deprivation. Previous genetic evidence suggested that SNF2 and SNF5 have functionally related roles. We cloned both genes by complementation and showed that the cloned DNA was tightly linked to the corresponding chromosomal locus. Both genes in multiple copy complemented only the cognate snf mutation. The SNF2 gene encodes a 5.7-kilobase RNA, and the SNF5 gene encodes a 3-kilobase RNA. Both RNAs contained poly(A) and were present in low abundance. Neither was regulated by glucose repression, and the level of SNF2 RNA was not dependent on SNF5 function or vice versa. Disruption of either gene at its chromosomal locus still allowed low-level derepression of secreted invertase activity, suggesting that these genes are required for high-level expression but are not directly involved in regulation. Further evidence was the finding that snf2 and snf5 mutants failed to derepress acid phosphatase, which is not regulated by glucose repression. The SNF2 and SNF5 functions were required for derepression of SUC2 mRNA.
机译：SNF2和SNF5基因是SUC2和其他酿酒酵母响应葡萄糖剥夺的葡萄糖可抑制基因的阻遏所必需的。以前的遗传证据表明SNF2和SNF5具有功能相关的作用。我们通过互补克隆了两个基因，并表明克隆的DNA与相应的染色体基因座紧密相连。多个拷贝中的两个基因仅互补同源snf突变。 SNF2基因编码一个5.7碱基碱基的RNA，而SNF5基因编码一个3碱基碱基的RNA。这两个RNA都含有poly（A），并且含量很低。两者均不受葡萄糖抑制的调节，并且SNF2 RNA的水平不依赖于SNF5的功能，反之亦然。任一基因在其染色体基因座处的破坏仍然允许分泌型转化酶活性的低水平抑制，这表明这些基因是高水平表达所必需的，但不直接参与调控。进一步的证据是发现snf2和snf5突变体无法抑制酸性磷酸酶，而该酶不受葡萄糖阻抑作用的调节。 SNF2和SNF5功能是SUC2 mRNA抑制所必需的。

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