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首页> 外文期刊>Molecular and Cellular Biology >Structure of the protein encoded by the chicken proto-oncogene c-myb.
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Structure of the protein encoded by the chicken proto-oncogene c-myb.

机译：鸡原癌基因c-myb编码的蛋白质的结构。

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The retroviral oncogene v-myb arose by transduction of the chicken proto-oncogene c-myb. We isolated and sequenced cDNA that represents the entire coding domain of chicken c-myb. By transcribing the cDNA into mRNA in vitro and then translating the RNA, we were able to document the integrity of the cDNA and to identify the codon responsible for initiation of translation from c-myb. Two different alleles of v-myb are extant, one in the genome of avian myeloblastosis virus (AMV) and the other in the genome of erythroblastosis virus 26 (E26V). The proteins encoded by the AMV and E26V alleles of v-myb differ from the product of c-myb in three ways: at their amino termini, they lack 71 and 80 amino acids respectively; at their carboxy termini, they are deficient in 199 and 278 residues; and 11 substitutions of amino acids are scattered throughout the product of AMV allele, whereas the product of the E26V allele contains only a single substitution. The structural origins of tumorigenicity by v-myb and the biological functions of c-myb remain enigmatic. The findings and molecular clones described here should now permit a systematic exploration of these enigmas.
机译：逆转录病毒癌基因v-myb通过转导鸡原癌基因c-myb产生。我们分离并测序了代表鸡c-myb整个编码域的cDNA。通过在体外将cDNA转录为mRNA，然后翻译RNA，我们能够证明cDNA的完整性，并鉴定出负责引发c-myb翻译的密码子。存在v-myb的两个不同等位基因，一个在禽成纤维细胞病病毒（AMV）的基因组中，另一个在成红细胞病病毒26（E26V）的基因组中。 v-myb的AMV和E26V等位基因编码的蛋白质在三个方面不同于c-myb的产物：在其氨基末端分别缺少71和80个氨基酸。在其羧基末端，它们缺少199和278个残基;氨基酸的11个取代分散在整个AMV等位基因的产物中，而E26V等位基因的产物仅包含一个取代。 v-myb致瘤性的结构起源和c-myb的生物学功能仍然是个谜。现在，这里描述的发现和分子克隆应该可以对这些谜团进行系统的探索。

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