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首页> 外文期刊>Molecular and Cellular Biology >Augmentation of c-fos mRNA expression by activators of protein kinase C in fresh, terminally differentiated resting macrophages.
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Augmentation of c-fos mRNA expression by activators of protein kinase C in fresh, terminally differentiated resting macrophages.

机译：在新鲜的，终末分化的静止巨噬细胞中，蛋白激酶C的激活剂增强了c-fos mRNA的表达。

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Expression of c-fos mRNA was investigated in fresh, normal peritoneal macrophages (M phi), which are terminally differentiated, nonproliferating cells. The levels of c-fos mRNA were dramatically increased by stimulation with phorbol myristate acetate (PMA), calcium ionophore, or 1-oleoyl-2-acetoyl glycerol (OAG). Induction of c-fos mRNA by all the above agents followed similar kinetics, with a peak of mRNA 30 min after stimulation. These results demonstrate that c-fos mRNA can be augmented in fresh, terminally differentiated cells. Since the stimuli increasing c-fos mRNA are direct or indirect activators of protein kinase C, our data suggest that in M phi c-fos mRNA is controlled by protein kinase C activation. PMA, calcium ionophore, and OAG were biologically active in M phi. PMA and calcium ionophore induced respiratory burst and tumoricidal activity, respectively, whereas OAG and PMA were chemotactic for M phi. Interferons beta and gamma, potent M phi activators eliciting tumoricidal activity, did not alter the levels of c-fos mRNA. These results indicate that c-fos mRNA augmentation is a stimulus-specific rather than a function-specific response connected to activation of protein kinase C.
机译：在新鲜的正常腹膜巨噬细胞（M phi）中研究了c-fos mRNA的表达，它们是终末分化的非增殖细胞。通过用佛波肉豆蔻酸酯乙酸酯（PMA），钙离子载体或1-油酰基-2-乙酰酰基甘油（OAG）刺激，可显着提高c-fos mRNA的水平。所有上述试剂对c-fos mRNA的诱导遵循相似的动力学，在刺激后30分钟达到mRNA的峰值。这些结果表明，c-fos mRNA可以在新鲜的终末分化细胞中增强。由于刺激性增加的c-fos mRNA是蛋白激酶C的直接或间接激活剂，因此我们的数据表明在M phi中，c-fos mRNA受蛋白激酶C激活的控制。 PMA，钙离子载体和OAG在M phi中具有生物活性。 PMA和钙离子载体分别诱导呼吸爆发和杀肿瘤活性，而OAG和PMA是M phi的趋化性。干扰素β和γ是有效的M phi激活剂，可引起杀伤活性，但并未改变c-fos mRNA的水平。这些结果表明，c-fos mRNA的增加是与蛋白激酶C激活有关的刺激特异性应答，而不是功能特异性应答。

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