• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular and Cellular Biology >Expression of the Aplysia californica rho gene in Escherichia coli: purification and characterization of its encoded p21 product.
【24h】

Expression of the Aplysia californica rho gene in Escherichia coli: purification and characterization of its encoded p21 product.

机译：Aplysia californica rho基因在大肠杆菌中的表达：纯化和鉴定其编码的p21产物。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A new family of highly conserved genes, designated rho, has recently been isolated and characterized (P. Madaule and R. Axel, Cell 41:31-40, 1985). These genes have been found in Saccharomyces cerevisiae, Drosophila melanogaster, rats, and humans, and their 21,000-dalton products are highly homologous. The rho p21 protein shares 35% amino acid homology with the Harvey ras p21 protein and on this basis has been proposed to be a G protein. We expressed the Aplysia californica rho gene in Escherichia coli and purified its p21 protein to more than 90% purity. The availability of the rho protein in high quantities made it possible to establish its high affinity for guanine nucleotides. The rho p21 protein had nucleotide-binding properties similar to those of the ras p21 protein. However, a comparison of these proteins revealed some important differences regarding their specificities and affinities. Finally, the rho p21 protein had GTPase activity almost identical to that of a normal ras p21 protein, the rates being 0.106 and 0.105 mol/min per mol of p21, respectively. Thus, the results suggest that the degree of homology found between the ras and rho genes products most likely is related to the conservation of sequences relevant to their ability to bind and hydrolyze guanine nucleotides. The fact that the rho p21 protein binds and hydrolyzes GTP strongly suggests that it is a G protein with a potential regulatory function conserved in evolution.
机译：最近分离并鉴定了新的高度保守的基因家族，称为rho，（P.Madaule和R.Axel，Cell 41：31-40，1985）。这些基因已经在酿酒酵母，果蝇果蝇，大鼠和人类中发现，它们的21,000道尔顿产物高度同源。 rho p21蛋白与Harvey ras p21蛋白具有35％的氨基酸同源性，并据此提出了G蛋白。我们在大肠杆菌中表达了Aplysia californica rho基因，并纯化了其p21蛋白至90％以上的纯度。 rho蛋白的大量使用使建立对鸟嘌呤核苷酸的高亲和力成为可能。 rho p21蛋白具有与ras p21蛋白相似的核苷酸结合特性。但是，对这些蛋白质进行比较后发现，它们的特异性和亲和力存在一些重要差异。最后，rho p21蛋白具有的GTPase活性几乎与正常ras p21蛋白相同，其速率分别为每摩尔p21 0.106和0.105 mol / min。因此，结果表明在ras和rho基因产物之间发现的同源性程度最可能与与它们结合和水解鸟嘌呤核苷酸的能力有关的序列的保守性有关。 rho p21蛋白结合并水解GTP的事实强烈表明，它是一种具有在进化中保守的潜在调节功能的G蛋白。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号