Isolation of a cDNA clone for human threonyl-tRNA synthetase: amplification of the structural gene in borrelidin-resistant cell lines.

K J Kontis; S M Arfin

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Isolation of a cDNA clone for human threonyl-tRNA synthetase: amplification of the structural gene in borrelidin-resistant cell lines.

机译：人苏糖基-tRNA合成酶的cDNA克隆的分离：增强抗硼瑞林蛋白的细胞系中结构基因的扩增。

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A cDNA for threonyl-tRNA synthetase was isolated from a human placental cDNA lambda gt11 expression library by immunological screening, and its identity was confirmed by hybrid-selected mRNA translation. With this cDNA used as a hybridization probe, borrelidin-resistant Chinese hamster ovary cells that overproduced threonyl-tRNA synthetase were shown to have increased levels of threonyl-tRNA synthetase mRNA and gene sequences. Amplification of the gene did not appear to have been accompanied by any major structural reorganizations.

机译：通过免疫筛选从人胎盘lambda gt11表达文库中分离出苏氨酰-tRNA合成酶的cDNA，并通过杂合选择的mRNA翻译来确认其同一性。用该cDNA作为杂交探针，显示出过量生产苏糖基-tRNA合成酶的抗硼瑞林的中国仓鼠卵巢细胞具有增加的苏糖基-tRNA合成酶mRNA和基因序列水平。该基因的扩增似乎没有伴随任何重大的结构重组。

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《Molecular and Cellular Biology》 |1989年第5期|共7页
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K J Kontis; S M Arfin;
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中图分类细胞生物学;
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1. Subtractive cDNA cloning using oligo(dt)30-latex and PCR: isolation of cDNA clones specific to undifferentiated human embryonal carcinoma cells [J] . Eiji Hara, Kinichiro Oda, Souei Sekiya, Nucleic acids research . 1991,第25期

机译：使用oligo（dt）30-latex和PCR的减法cDNA克隆：分离未分化的人类胚胎癌细胞特异的cDNA克隆
2. The cloning and nucleotide sequence of cDNA for an amplified glutamine synthetase gene from the Chinese hamster [J] . A. Hussain, A. Lyons, B. McIntosh, Nucleic acids research . 1986,第2期

机译：中国仓鼠扩增的谷氨酰胺合成酶基因cDNA的克隆与核苷酸序列
3. The cloning and nucleotide sequence of cDNA for an amplified glutamine synthetase gene from the Chinese hamster [J] . A. Hussain, A. Lyons, B. McIntosh, Nucleic acids research . 1986,第2期

机译：中国仓鼠扩增的谷氨酰胺合成酶基因cDNA的克隆与核苷酸序列
4. Expression and amplification of glutamine synthetase gene for constructing ammonia-metabolizing cell lines in hybrid bioartificial liver support system [C] . Takeshi Omasa, Kazumi Kim, Shin-ya Hiramatsu, Annual Meeting of the Japanese Association for Animal Cell Technology . 2002

机译：谷氨酰胺合成酶基因的表达及扩增在杂交生物肝支持系统中构建氨代谢细胞系
5. Isolation of a humancDNA for threonyl-tRNA synthetase: Amplification and regulation of the structural gene in Chinese hamster ovary cells. [D] . Kontis, Kris John. 1989

机译：苏糖基-tRNA合成酶的人类cDNA的分离：中国仓鼠卵巢细胞结构基因的扩增和调控。
6. Isolation of a cDNA clone for human threonyl-tRNA synthetase: amplification of the structural gene in borrelidin-resistant cell lines. [O] . K J Kontis, S M Arfin 1989

机译：人类苏氨酰-tRNA合成酶的cDNA克隆的分离：耐硼瑞林的细胞系中结构基因的扩增。
7. Isolation of a cDNA clone for human threonyl-tRNA synthetase: amplification of the structural gene in borrelidin-resistant cell lines. [O] . Kontis, K J, Arfin, S M 1989

机译：人类苏氨酰-tRNA合成酶的cDNA克隆的分离：耐硼瑞林的细胞系中结构基因的扩增。
8. Isolation of cDNA Clones Encoding the 20K Non-Glycosylated Polypeptide Chain of the Human T-Cell Receptor/T3 Complex [R] . Gold, D. P., Puck, J. M., Pettey, C. L., 1986

机译：编码人T细胞受体/ T3复合物的20K非糖基化多肽链的cDNa克隆的分离

Isolation of a cDNA clone for human threonyl-tRNA synthetase: amplification of the structural gene in borrelidin-resistant cell lines.

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