A 36-kilodalton cellular transcription factor mediates an indirect interaction of human T-cell leukemia/lymphoma virus type I TAX1 with a responsive element in the viral long terminal repeat.

S J Marriott; P F Lindholm; K M Brown; S D Gitlin; J F Duvall; M F Radonovich; J N Brady

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A 36-kilodalton cellular transcription factor mediates an indirect interaction of human T-cell leukemia/lymphoma virus type I TAX1 with a responsive element in the viral long terminal repeat.

机译：36千达尔顿细胞转录因子介导人T细胞白血病/淋巴瘤病毒I TAX1与病毒长末端重复序列中的应答元件间接相互作用。

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The human T-cell leukemia/lymphoma virus type I (HTLV-I) trans activator, TAX1, interacts indirectly with a TAX1-responsive element, TRE-2, located at positions -117 to -163 in the viral long terminal repeat. This report describes the characterization of a 36-kilodalton (kDa) protein identified in HeLa nuclear extract which mediates the interaction of TAX1 with TRE-2. Purification of the protein was achieved by zinc chelate chromatography and preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The renatured 36-kDa protein bound specifically to a TRE-2 oligonucleotide but not to nonfunctional base substitution mutant probes in a gel retardation assay. Renatured proteins of differing molecular weights were unable to form this complex. In addition, the 36-kDa protein specifically activated transcription from the HTLV-I promoter in vitro. Purified TAX1 protein formed a complex with the TRE-2 oligonucleotide in the presence of the 36-kDa protein, suggesting that indirect interaction of TAX1 with the viral long terminal repeat may be one of the mechanisms by which HTLV-I transcription is regulated.

机译：I型人T细胞白血病/淋巴瘤病毒（HTLV-1）反式激活剂TAX1与TAX-1反应元件TRE-2间接相互作用，该元件位于病毒长末端重复序列中的-117至-163位。该报告描述了在HeLa核提取物中鉴定出的36千达尔顿（kDa）蛋白的特征，该蛋白介导TAX1与TRE-2的相互作用。通过螯合锌层析和制备型十二烷基硫酸钠-聚丙烯酰胺凝胶电泳实现蛋白质的纯化。在凝胶阻滞分析中，经过变性的36 kDa蛋白与TRE-2寡核苷酸特异性结合，但与非功能性碱基取代突变探针没有特异性结合。分子量不同的变性蛋白质无法形成这种复合物。另外，该36-kDa蛋白在体外特异性激活了HTLV-1启动子的转录。纯化的TAX1蛋白在36 kDa蛋白存在下与TRE-2寡核苷酸形成复合物，这表明TAX1与病毒长末端重复序列的间接相互作用可能是HTLV-1转录调控的机制之一。

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《Molecular and Cellular Biology》 |1990年第8期|共10页
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S J Marriott; P F Lindholm; K M Brown; S D Gitlin; J F Duvall; M F Radonovich; J N Brady;
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中图分类细胞生物学;
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1. Indirect binding of human T-cell leukemia virus type I tax1 to a responsive element in the viral long terminal repeat. [J] . S J Marriott, I Boros, J F Duvall, Molecular and Cellular Biology . 1989,第10期

机译：I型人T细胞白血病病毒tax1与病毒长末端重复序列中的应答元件间接结合。
2. Genetic characterization of transactivation of the human T-cell leukemia virus type 1 promoter: Binding of Tax to Tax-responsive element 1 is mediated by the cyclic AMP-responsive members of the CREB/ATF family of transcription factors. [J] . F Bantignies, R Rousset, C Desbois, Molecular and Cellular Biology . 1996,第5期

机译：人类T细胞白血病病毒1型启动子反式激活的遗传特性：CREB / ATF转录因子家族的AMP响应成员介导Tax与Tax响应元件1的结合。
3. Differential patterns of human cytomegalovirus gene expression in various T-cell lines carrying human T-cell leukemia-lymphoma virus type I: role of Tax-activated cellular transcription factors. [J] . Beck Z, Bacsi A, Liu X, Journal of Medical Virology . 2003,第1期

机译：携带I型人T细胞白血病-淋巴瘤病毒的各种T细胞系中人巨细胞病毒基因表达的差异模式：税激活细胞转录因子的作用。
4. Transcriptional regulation of human immunodeficiency virus type 1 via the cis-acting binding elements in the long terminal repeat. [D] . Dahiya, Satinder. 2014

机译：通过长末端重复序列中的顺式作用结合元件对人类1型免疫缺陷病毒进行转录调节。
5. A 36-kilodalton cellular transcription factor mediates an indirect interaction of human T-cell leukemia/lymphoma virus type I TAX1 with a responsive element in the viral long terminal repeat. [O] . S J Marriott, P F Lindholm, K M Brown, 1990

机译：36千达尔顿细胞转录因子介导人T细胞白血病/淋巴瘤病毒I TAX1与病毒长末端重复序列中的应答元件间接相互作用。
6. A 36-kilodalton cellular transcription factor mediates an indirect interaction of human T-cell leukemia/lymphoma virus type I TAX1 with a responsive element in the viral long terminal repeat. [O] . Marriott, S J, Lindholm, P F, Brown, K M, 1990

机译：36千达尔顿细胞转录因子介导人T细胞白血病/淋巴瘤病毒I TAX1与病毒长末端重复序列中的应答元件间接相互作用。

A 36-kilodalton cellular transcription factor mediates an indirect interaction of human T-cell leukemia/lymphoma virus type I TAX1 with a responsive element in the viral long terminal repeat.

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