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首页> 外文期刊>Molecular and Cellular Biology >The ARS consensus sequence is required for chromosomal origin function in Saccharomyces cerevisiae.
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The ARS consensus sequence is required for chromosomal origin function in Saccharomyces cerevisiae.

机译：ARS共有序列是酿酒酵母中染色体起源功能所必需的。

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摘要

Replication origins have been mapped to positions that coincide, within experimental error (several hundred base pairs), with ARS elements. To determine whether the DNA sequences required for ARS function on plasmids are required for chromosomal origin function, the chromosomal copy of ARS306 was deleted and the chromosomal copy of ARS307 was replaced with mutant derivatives of ARS307 containing single point mutations in domain A within the ARS core consensus sequence. The chromosomal origin function of these derivatives was assayed by two-dimensional agarose gel electrophoresis. Deletion of ARS306 deleted the associated replication origin. The effects on chromosomal origin function of mutations in domain A paralleled their effects on ARS function, as measured by plasmid stability. These results demonstrate that chromosomal origin function is a property of the ARS element itself.
机译：复制起点已映射到在实验误差（几百个碱基对）内与ARS元素重合的位置。为了确定质粒的ARS功能所需的DNA序列是否是染色体起源功能所必需的，删除了ARS306的染色体副本，并用ARS307的突变体衍生物替换了ARS307的染色体副本，该衍生物包含ARS核心内域A中的单点突变共有序列。这些衍生物的染色体起源功能通过二维琼脂糖凝胶电泳测定。删除ARS306会删除关联的复制源。结构域A中的突变对染色体起源功能的影响与它们对ARS功能的影响平行，如通过质粒稳定性测量的。这些结果表明，染色体起源功能是ARS元素本身的一个属性。

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