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首页> 外文期刊>Molecular and Cellular Biology >Segments of the POU domain influence one another's DNA-binding specificity.
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Segments of the POU domain influence one another's DNA-binding specificity.

机译：POU结构域的片段影响彼此的DNA结合特异性。

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摘要

The ubiquitously expressed mammalian POU-domain protein Oct-1 specifically recognizes two classes of cis-acting regulatory elements that bear little sequence similarity, the octamer motif ATGCAAAT and the TAATGARAT motif. The related pituitary-specific POU protein Pit-1 also recognizes these two motifs but, unlike Oct-1, binds preferentially to the TAATGARAT motif. Yet in our assay, Pit-1 still binds octamer elements better than does the octamer motif-binding protein Oct-3. The POU domain is responsible for recognizing these diverse regulatory sequences through multiple DNA contacts that include the two POU subdomains, the POU-specific region, and the POU homeodomain. The DNA-binding properties of 10 chimeric POU domains, in which different POU-domain segments are derived from either Oct-1 or Pit-1, reveal a high degree of structural plasticity; these hybrid proteins all bind DNA well and frequently bind particular sites better than does either of the parental POU domains. In these chimeric POU domains, the POU-specific A and B boxes and the hypervariable POU linker can influence DNA-binding specificity. The surprising result is that the influence a particular segment has on DNA-binding specificity can be greatly affected by the origin of other segments of the POU domain and the sequence of the binding site. Thus, the broad but selective DNA-binding specificity of Oct-1 is conferred both by multiple DNA contacts and by dynamic interactions within the DNA-bound POU domain.
机译：普遍表达的哺乳动物POU域蛋白Oct-1特异性识别两类几乎没有序列相似性的顺式作用调控元件，即八聚体基序ATGCAAAT和TAATGARAT基序。相关的垂体特异性POU蛋白Pit-1也可以识别这两个基序，但与Oct-1不同，它优先结合TAATGARAT基序。然而，在我们的测定中，Pit-1仍然比八聚体基序结合蛋白Oct-3更好地结合八聚体元素。 POU域负责通过多个DNA接触识别这些不同的调控序列，这些DNA接触包括两个POU子域，POU特异性区域和POU同源域。 10个嵌合的POU域的DNA结合特性显示了高度的结构可塑性，其中不同的POU结构域片段来自Oct-1或Pit-1。这些杂合蛋白均能很好地结合DNA，并且经常比两个亲本POU域都更好地结合特定位点。在这些嵌合的POU域中，特定于POU的A和B框以及高变POU接头可影响DNA结合特异性。令人惊讶的结果是，特定片段对DNA结合特异性的影响可能会受到POU结构域其他片段的起源和结合位点序列的极大影响。因此，Oct-1的广泛但选择性的DNA结合特异性是由多个DNA接触以及与DNA结合的POU域内的动态相互作用所赋予的。

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