...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular and Cellular Biology >A mouse oocyte-specific protein that binds to a region of mZP3 promoter responsible for oocyte-specific mZP3 gene expression.
【24h】

A mouse oocyte-specific protein that binds to a region of mZP3 promoter responsible for oocyte-specific mZP3 gene expression.

机译：小鼠卵母细胞特异性蛋白，与负责卵母细胞特异性mZP3基因表达的mZP3启动子区域结合。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The gene encoding mZP3, the mouse sperm receptor, is expressed exclusively in growing oocytes during oogenesis. To investigate the molecular basis of oocyte-specific mZP3 gene expression, we generated several lines of mice harboring a transgene that contains 470 bp of mZP3 gene 5'-flanking sequence (nucleotides -470 to +10) fused to the firefly luciferase gene coding region. Three of four expressing transgenic lines exhibited luciferase activity only in growing oocytes, suggesting that the 470-bp fragment is sufficient to direct Iocyte-specific expression of the luciferase gene. Results of DNase I footprinting and gel mobility shift assays suggested the presence of an ovary-specific protein that binds to a small region (nucleotides-99 to -86) within the 470-bp fragment of the mZP3 promoter, with 5'-G(G/A)T(G/A)A-3' representing the minimal sequence required for binding. Southwestern (DNA-protein) gel blots revealed the presence of an oocyte-specific, approximately 60,000-Mr protein, called OSP-1, that binds to the minimal sequence. Changes in levels of OSP-1 during oogenesis and early cleavage are consistent with the pattern of mZP3 gene expression during these developmental stages in mice. Therefore, OSP-1 may be a mammalian oocyte-specific transcription factor involved in regulating oocyte-specific mZP3 gene expression.
机译：编码小鼠精子受体mZP3的基因在卵子发生过程中仅在生长的卵母细胞中表达。为了研究卵母细胞特异性mZP3基因表达的分子基础，我们制备了几只小鼠，这些小鼠的转基因包含与萤火虫荧光素酶基因编码区融合的470 bp mZP3基因5'侧翼序列（核苷酸-470至+10） 。四个表达转基因品系中的三个仅在生长的卵母细胞中显示萤光素酶活性，这表明470 bp的片段足以指导萤光素酶基因的细胞特异性表达。 DNase I足迹和凝胶迁移率迁移分析的结果表明，卵巢特异性蛋白的存在与mZP3启动子的470-bp片段中的小区域（核苷酸-99至-86）结合，具有5'-G（ G / A）T（G / A）A-3'表示结合所需的最小序列。西南（DNA-蛋白质）凝胶印迹显示，存在卵母细胞特异性，约60,000-Mr的蛋白质，称为OSP-1，与最小序列结合。在卵子发生和早期卵裂期间OSP-1水平的变化与小鼠在这些发育阶段的mZP3基因表达模式一致。因此，OSP-1可能是哺乳动物卵母细胞特异性转录因子，参与调节卵母细胞特异性mZP3基因表达。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号