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首页> 外文期刊>Molecular and Cellular Biology >The protein encoded by a growth arrest-specific gene (gas6) is a new member of the vitamin K-dependent proteins related to protein S, a negative coregulator in the blood coagulation cascade.
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The protein encoded by a growth arrest-specific gene (gas6) is a new member of the vitamin K-dependent proteins related to protein S, a negative coregulator in the blood coagulation cascade.

机译：由生长停滞特异基因（gas6）编码的蛋白质是与蛋白质S有关的维生素K依赖性蛋白质的新成员，蛋白质S是血液凝固级联反应中的负调节剂。

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A set of growth arrest-specific genes (gas) whose expression is negatively regulated after serum induction has previously been described (C. Schneider, R. M. King, and L. Philipson, Cell 54:787-793, 1988). The detailed analysis of one of them, gas6, is reported here, gas6 mRNA (2.6 kb) is abundantly expressed in serum-starved (48 h in 0.5% fetal calf serum) NIH 3T3 cells but decreases dramatically after fetal calf serum or basic fibroblast growth factor stimulation. The human homolog of gas6 was also cloned and sequenced, revealing a high degree of homology and a similar pattern of expression in IMR90 human fibroblasts. Computer analysis of the protein encoded by murine and human gas6 cDNAs showed significant homology (43 and 44% amino acid identity, respectively) to human protein S, a negative coregulator in the blood coagulation pathway. By using an anti-human Gas6 monospecific affinity-purified antibody, we show that the biosynthetic level of human Gas6 fully reflects mRNA expression in IMR90 human fibroblasts. This finding thus defines a new member of vitamin K-dependent proteins that is expressed in many human and mouse tissues and may be involved in the regulation of a protease cascade relevant in growth regulation.
机译：先前已经描述了一组生长停滞特异性基因（气体），其表达在血清诱导后被负调节（C.Schneider，R.M.King，和L.Philipson，Cell 54：787-793，1988）。这里报道了其中一种气体gas6的详细分析，gas6 mRNA（2.6 kb）在饥饿的血清中（0.5％胎牛血清中48小时）在NIH 3T3细胞中大量表达，但在胎牛血清或碱性成纤维细胞中会急剧下降生长因子刺激。还克隆了gas6的人类同源物并进行了测序，揭示了IMR90人类成纤维细胞中的高度同源性和相似的表达模式。对鼠和人gas6 cDNA编码的蛋白质的计算机分析显示，与人蛋白质S（血液凝结途径中的负调节剂）具有显着同源性（分别为43％和44％氨基酸同一性）。通过使用抗人Gas6单特异性亲和纯化的抗体，我们显示人Gas6的生物合成水平完全反映了IMR90人成纤维细胞中的mRNA表达。因此，该发现定义了维生素K依赖性蛋白的新成员，其在许多人和小鼠组织中表达，并且可能参与与生长调节相关的蛋白酶级联的调节。

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