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首页> 外文期刊>Molecular and Cellular Biology >Characterization of a family of related cellular transcription factors which can modulate human immunodeficiency virus type 1 transcription in vitro.
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Characterization of a family of related cellular transcription factors which can modulate human immunodeficiency virus type 1 transcription in vitro.

机译：表征一系列相关细胞转录因子的因子，它们可以在体外调节人免疫缺陷病毒1型的转录。

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LBP-1 is a cellular protein which binds strongly to sequences around the human immunodeficiency virus type 1 (HIV-1) initiation site and weakly over the TATA box. We have previously shown that LBP-1 represses HIV-1 transcription by inhibiting the binding of TFIID to the TATA box. Four similar but distinct cDNAs encoding LBP-1 (LBP-1a, -b, -c, and -d) have been isolated. These are products of two related genes, and each gene encodes two alternatively spliced products. Comparison of the amino acid sequence of LBP-1 with entries in the available protein data bases revealed the identity of LBP-1c to alpha-CP2, an alpha-globin transcription factor. These proteins are also homologous to Drosophila melanogaster Elf-1/NTF-1, an essential transcriptional activator that functions during Drosophila embryogenesis. Three of the recombinant LBP-1 isoforms show DNA binding specificity identical to that of native LBP-1 and bind DNA as a multimer. In addition, antisera raised against recombinant LBP-1 recognize native LBP-1 from HeLa nuclear extract. Functional analyses in a cell-free transcription system demonstrate that recombinant LBP-1 specifically represses transcription from a wild-type HIV-1 template but not from an LBP-1 mutant template. Moreover, LBP-1 can function as an activator both in vivo and in vitro, depending on the promoter context. Interestingly, one isoform of LBP-1 which is missing the region of the Elf-1/NTF-1 homology is unable to bind DNA itself and, presumably through heteromer formation, inhibits binding of the other forms of LBP-1, suggesting that it may function as a dominant negative regulator.
机译：LBP-1是一种细胞蛋白，可与1型人类免疫缺陷病毒（HIV-1）起始位点周围的序列牢固结合，而与TATA盒的结合弱。先前我们已经证明LBP-1通过抑制TFIID与TATA盒的结合来抑制HIV-1转录。已分离出四个相似但截然不同的编码LBP-1的cDNA（LBP-1a，-b，-c和-d）。这些是两个相关基因的产物，每个基因编码两个交替剪接的产物。将LBP-1的氨基酸序列与可用蛋白质数据库中的条目进行比较，发现LBP-1c与α-珠蛋白转录因子alpha-CP2的身份相同。这些蛋白质也与果蝇果蝇Elf-1 / NTF-1同源，果蝇是在果蝇胚胎发生过程中起作用的必需转录激活因子。重组LBP-1亚型中的三个显示出与天然LBP-1相同的DNA结合特异性，并以多聚体形式结合DNA。此外，针对重组LBP-1的抗血清可识别来自HeLa核提取物的天然LBP-1。无细胞转录系统中的功能分析表明重组LBP-1特异性抑制野生型HIV-1模板的转录，而不抑制LBP-1突变体模板的转录。此外，根据启动子的情况，LBP-1可以在体内和体外起激活剂的作用。有趣的是，缺少Elf-1 / NTF-1同源性区域的LBP-1的一种同工型无法结合DNA本身，并且大概是通过异源异构体形成，抑制了其他形式的LBP-1的结合，表明它可能起主导作用的负调节剂作用。

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