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首页> 外文期刊>Molecular and Cellular Biology >Characterization of a delayed early serum response region.
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Characterization of a delayed early serum response region.

机译：延迟血清早期反应区的特征。

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The proliferin (PLF) gene promoter provides a relatively simple model system for the study of growth-regulated gene expression in mouse cells. The promoter elements required for this serum-induced regulation have been identified and include an AP-1 site as well as an adjacent element comprised of three imperfect repeats that are similar in sequence to the simian virus 40 (SV40) Sph motif. Distinct protein complexes bound independently to the AP-1 and Sph elements, and both of these juxtaposed sites could be occupied simultaneously. Furthermore, serum stimulation of mouse fibroblasts resulted in similar increases in protein binding to the AP-1 and Sph elements. Consistent with this increase in AP-1 and Sph binding activity, the PLF AP-1 and Sph elements were independently able to confer serum responsiveness to a minimal promoter, and together these two elements acted synergistically in response to serum. Although several members of the AP-1 family were able to activate the PLF gene promoter in transient cotransfection experiments, the predominant AP-1 components interacting with the PLF gene promoter in serum-stimulated cells were Fra-1, JunB, and JunD. Analysis of the Sph element revealed that mutation of Sph repeats I or III abolished serum responsiveness of the PLF gene promoter, and mutation of Sph repeat III decreased protein binding to this element. Although the Sph element is similar in sequence to the SV40 element, the PLF Sph-binding factor is distinct from TEF-1, the factor that binds to the SV40 Sph motif.
机译：proliferin（PLF）基因启动子提供了一个相对简单的模型系统，用于研究小鼠细胞中生长调节基因的表达。已经确定了这种血清诱导的调控所需的启动子元件，包括一个AP-1位点以及一个相邻的元件，该元件由三个不完善的重复序列组成，这些重复序列与猿猴病毒40（SV40）Sph基序相似。不同的蛋白质复合物独立地绑定到AP-1和Sph元素，并且这两个并列的位点可以同时被占用。此外，小鼠成纤维细胞的血清刺激导致蛋白质与AP-1和Sph元素的结合增加。与AP-1和Sph结合活性的这种增加一致，PLF AP-1和Sph元素能够独立地赋予血清对最小启动子的响应性，并且这两个元素共同响应血清。尽管AP-1家族的几个成员能够在瞬时共转染实验中激活PLF基因启动子，但在血清刺激的细胞中与PLF基因启动子相互作用的主要AP-1成分是Fra-1，JunB和JunD。对Sph元件的分析表明，Sph重复序列I或III的突变消除了PLF基因启动子的血清反应性，并且Sph重复序列III的突变降低了蛋白质与该元件的结合。尽管Sph元素在序列上与SV40元素相似，但PLF Sph结合因子与TEF-1不同，TEF-1是与SV40 Sph基序结合的因子。

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