• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular and Cellular Biology >Conserved intron elements repress splicing of a neuron-specific c-src exon in vitro.
【24h】

Conserved intron elements repress splicing of a neuron-specific c-src exon in vitro.

机译：保守的内含子元件在体外抑制神经元特异性c-src外显子的剪接。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The neuron-specific N1 exon of the mouse c-src transcript is normally skipped in nonneuronal cells. In this study, we examined the sequence requirements for the exclusion of this exon in nonneuronal HeLa cell nuclear extracts. We found that the repression of the N1 exon is mediated by specific intron sequences that flank the N1 exon. Mutagenesis experiments identified conserved CUCUCU elements within these intron regions that are required for the repression of N1 splicing. The addition of an RNA competitor containing the upstream regulatory sequence to the HeLa extract induced splicing of the intron downstream of N1, indicating that the competitor sequence binds to splicing repressor proteins. The similarities between this mechanism for src splicing repression and the repression of other regulated exons point to a common role of exon-spanning interactions in splicing repression.
机译：鼠标c-src转录本的神经元特异性N1外显子通常在非神经元细胞中被跳过。在这项研究中，我们检查了在非神经元HeLa细胞核提取物中排除该外显子的序列要求。我们发现，N1外显子的抑制是由位于N1外显子侧面的特定内含子序列介导的。诱变实验确定了这些内含子区域内保守NCU剪接所需的保守CUCUCU元件。向HeLa提取物中添加一个含有上游调控序列的RNA竞争子，可诱导N1下游内含子的剪接，表明该竞争子序列与剪接阻遏蛋白结合。 src剪接抑制的这种机制与其他受调控外显子的抑制之间的相似之处表明，跨剪接作用在剪接抑制中具有共同的作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号