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首页> 外文期刊>Molecular and Cellular Biology >Bending DNA can repress a eukaryotic basal promoter and inhibit TFIID binding.
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Bending DNA can repress a eukaryotic basal promoter and inhibit TFIID binding.

机译：弯曲的DNA可以抑制真核基础启动子并抑制TFIID结合。

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Previous studies indicated that repression by eve involves cooperative DNA binding and leads to the formation of a DNA loop which encompasses the DNA sequences normally bound by the RNA polymerase II general transcription factors. To test the general principle of whether bending of a basal promoter sequence can contribute directly to repression of transcription, a minicircle template of 245 bp was used. In a purified transcription system, transcription from the minicircular DNA is greatly reduced compared with that from the identical DNA fragment in linear form. Transcription is also reduced when the minicircle contains a single-stranded nick, indicating that transcription is reduced because of DNA bending, rather than any constraint on supercoiling. We show that the reduced transcription from the minicircle in these experiments is not due to a reduced rate of elongation by RNA polymerase II. Rather, repression occurs, at least in part, because binding of the general transcription factor TFIID to the minicircle is strongly inhibited compared with binding to the linear DNA. We suggest that bending DNA may be a mechanism by which eukaryotic transcription may be regulated, by modulating the activity of the general transcription factors.
机译：先前的研究表明，前夕的抑制涉及合作的DNA结合，并导致形成DNA环，该环包含通常由RNA聚合酶II一般转录因子结合的DNA序列。为了测试基本启动子序列的弯曲是否可以直接促进转录抑制的一般原理，使用了一个245 bp的小环模板。在纯化的转录系统中，与线性形式的相同DNA片段相比，微环形DNA的转录大大减少。当微圆包含单链缺口时，转录也减少，这表明转录是由于DNA弯曲而不是对超螺旋的任何限制而减少的。我们显示，在这些实验中，从小圆环减少的转录不是由于RNA聚合酶II的伸长率降低。而是，至少部分地发生了抑制，因为与结合线性DNA相比，一般转录因子TFIID与小环的结合被强烈抑制。我们建议弯曲DNA可能是通过调节一般转录因子的活性来调节真核转录的机制。

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