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首页> 外文期刊>Molecular and Cellular Biology >Repression of the heat shock factor 1 transcriptional activation domain is modulated by constitutive phosphorylation.
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Repression of the heat shock factor 1 transcriptional activation domain is modulated by constitutive phosphorylation.

机译：热休克因子1转录激活域的抑制是通过组成型磷酸化来调节的。

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Heat shock transcription factor 1 (HSF1) is constitutively expressed in mammalian cells and negatively regulated for DNA binding and transcriptional activity. Upon exposure to heat shock and other forms of chemical and physiological stress, these activities of HSF1 are rapidly induced. In this report, we demonstrate that constitutive phosphorylation of HSF1 at serine residues distal to the transcriptional activation domain functions to repress transactivation. Tryptic phosphopeptide analysis of a collection of chimeric GAL4-HSF1 deletion and point mutants identified a region of constitutive phosphorylation encompassing serine residues 303 and 307. The significance of phosphorylation at serines 303 and 307 in the regulation of HSF1 transcriptional activity was demonstrated by transient transfection and assay of a chloramphenicol acetyltransferase reporter construct. Whereas the transfected wild-type GAL4-HSF1 chimera is repressed for transcriptional activity and derepressed by heat shock, mutation of serines 303 and 307 to alanine results in derepression to a high level of constitutive activity. Similar results were obtained with mutation of these serine residues in the context of full-length HSF1. These data reveal that constitutive phosphorylation of serines 303 and 307 has an important role in the negative regulation of HSF1 transcriptional activity at control temperatures.
机译：热休克转录因子1（HSF1）在哺乳动物细胞中组成性表达，并且对DNA结合和转录活性负调节。暴露于热冲击和其他形式的化学和生理应力后，HSF1的这些活性迅速被诱导。在此报告中，我们证明了在转录激活域远端的丝氨酸残基上，HSF1的组成型磷酸化可抑制反式激活。嵌合GAL4-HSF1缺失和点突变体集合的胰蛋白酶磷酸肽分析确定了组成性磷酸化区域，其中包含丝氨酸残基303和307。丝氨酸303和307磷酸化在调节HSF1转录活性中的重要性通过瞬时转染和氯霉素乙酰转移酶报告基因构建体的分析。转染的野生型GAL4-HSF1嵌合体的转录活性受到阻遏，而热激抑制了其转染，而丝氨酸303和307突变为丙氨酸会导致抑制力降低至高水平的组成活性。在全长HSF1的情况下，这些丝氨酸残基的突变获得了相似的结果。这些数据表明，在控制温度下，丝氨酸303和307的组成型磷酸化在HSF1转录活性的负调控中具有重要作用。

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