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首页> 外文期刊>Molecular and Cellular Biology >A basic helix-loop-helix-leucine zipper transcription complex in yeast functions in a signaling pathway from mitochondria to the nucleus.
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A basic helix-loop-helix-leucine zipper transcription complex in yeast functions in a signaling pathway from mitochondria to the nucleus.

机译：酵母中的基本螺旋-环-螺旋-亮氨酸拉链转录复合体在从线粒体到细胞核的信号通路中起作用。

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摘要

The expression of some nuclear genes in Saccharomyces cerevisiae, such as the CIT2 gene, which encodes a glyoxylate cycle isoform of citrate synthase, is responsive to the functional state of mitochondria. Previous studies identified a basic helix-loop-helix-leucine zipper (bHLH/Zip) transcription factor encoded by the RTG1 gene that is required for both basal expression of the CIT2 gene and its increased expression in respiratory-deficient cells. Here, we describe the cloning and characterization of RTG3, a gene encoding a 54-kDa bHLH/Zip protein that is also required for CIT2 expression. Rtg3p binds together with Rtg1p to two identical sites oriented as inverted repeats 28 bp apart in a regulatory upstream activation sequence element (UASr) in the CIT2 promoter. The core binding site for the Rtg1p-Rtg3p heterodimer is 5'-GGTCAC-3', which differs from the canonical E-box site, CANNTG, to which most other bHLH proteins bind. We demonstrate that both of the Rtg1p-Rtg3p binding sites in the UAS(r) element are required in vivo and act synergistically for CIT2 expression. The basic region of Rtg3p conforms well to the basic region of most bHLH proteins, whereas the basic region of Rtg1p does not. These findings suggest that the Rtg1p-Rtg3p complex interacts in a novel way with its DNA target sites.
机译：酿酒酵母中某些核基因的表达，例如编码柠檬酸合酶的乙醛酸循环同工型的CIT2基因，对线粒体的功能状态有反应。先前的研究确定了由RTG1基因编码的基本螺旋-环-螺旋-亮氨酸拉链（bHLH / Zip）转录因子，这是CIT2基因的基础表达及其在呼吸缺陷细胞中表达增加所必需的。在这里，我们描述了RTG3的克隆和特征，RTG3是编码54 kDa bHLH / Zip蛋白的基因，也是CIT2表达所必需的。 Rtg3p与Rtg1p结合到两个相同的位点，该位点在CIT2启动子的上游调节激活序列元件（UASr）中以间隔28 bp的反向重复序列定向。 Rtg1p-Rtg3p异二聚体的核心结合位点是5'-GGTCAC-3'，与大多数其他bHLH蛋白结合的标准E-box位点CANNTG不同。我们证明，UAS（r）元素中的两个Rtg1p-Rtg3p结合位点在体内都是必需的，并且对于CIT2表达具有协同作用。 Rtg3p的基本区域与大多数bHLH蛋白的基本区域非常吻合，而Rtg1p的基本区域则不然。这些发现表明，Rtg1p-Rtg3p复合物以一种新颖的方式与其DNA靶位点相互作用。

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