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首页> 外文期刊>Molecular and Cellular Biology >Modulated Expression of the Epidermal Growth Factor-Like Homeotic Protein dlk Influences Stromal-Cell–Pre-B-Cell Interactions, Stromal Cell Adipogenesis, and Pre-B-Cell Interleukin-7 Requirements
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Modulated Expression of the Epidermal Growth Factor-Like Homeotic Protein dlk Influences Stromal-Cell–Pre-B-Cell Interactions, Stromal Cell Adipogenesis, and Pre-B-Cell Interleukin-7 Requirements

机译：表皮生长因子样同源蛋白dlk的调节表达影响基质细胞-前B细胞相互作用，基质细胞成脂作用以及前B细胞白细胞介素7的要求。

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A close relationship exists between adipocyte differentiation of stromal cells and their capacity to support hematopoiesis. The molecular basis for this is unknown. We have studied whether dlk, an epidermal growth factor-like molecule that intervenes in adipogenesis and fetal liver hematopoiesis, affects both stromal cell adipogenesis and B-cell lymphopoiesis in an established pre-B-cell culture system. Pre-B-cell cultures require both soluble interleukin-7 (IL-7) and interactions with stromal cells to promote cell growth and prevent B-cell maturation or apoptosis. We found that BALB/c 3T3 fibroblasts express dlk and function as stromal cells. Transfection of these cells with antisense dlk decreased dlk expression and increased insulin-induced adipocytic differentiation. When antisense transfectants were used as stroma, IL-7 was no longer required to support the growth of pre-B cells and prevent maturation or apoptosis. Antisense dlk transfectants of S10 stromal cells also promoted pre-B-cell growth in the absence of IL-7. These results show that modulation of dlk on stromal cells can influence their adipogenesis and the IL-7 requirements of the pre-B cells growing in contact with them. These results indicate that dlk influences differentiation signals directed both to the stromal cells and to the lymphocyte precursors, suggesting that dlk may play an important role in the bone marrow hematopoietic environment.
机译：基质细胞的脂肪细胞分化与其支持造血功能之间存在密切关系。其分子基础是未知的。我们已经研究了dlk（一种介入脂肪形成和胎儿肝脏造血的表皮生长因子样分子）是否会影响已建立的B细胞前培养系统中的基质细胞脂肪生成和B细胞淋巴细胞生成。 B细胞前培养需要可溶性白介素7（IL-7）以及与基质细胞的相互作用，以促进细胞生长并防止B细胞成熟或凋亡。我们发现BALB / c 3T3成纤维细胞表达dlk并起基质细胞的作用。用反义dlk转染这些细胞会降低dlk表达并增加胰岛素诱导的脂肪细胞分化。当反义转染子用作基质时，不再需要IL-7来支持pre-B细胞的生长并防止其成熟或凋亡。在没有IL-7的情况下，S10基质细胞的反义dlk转染子也促进了前B细胞的生长。这些结果表明，dlk对基质细胞的调节可以影响它们的脂肪生成以及与之接触生长的pre-B细胞的IL-7需求。这些结果表明，dlk影响直接针对基质细胞和淋巴细胞前体的分化信号，这表明dlk可能在骨髓造血环境中起重要作用。

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