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首页> 外文期刊>Molecular and Cellular Biology >Mitochondrial Translation of Saccharomyces cerevisiae COX2 mRNA Is Controlled by the Nucleotide Sequence Specifying the Pre-Cox2p Leader Peptide
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Mitochondrial Translation of Saccharomyces cerevisiae COX2 mRNA Is Controlled by the Nucleotide Sequence Specifying the Pre-Cox2p Leader Peptide

机译：酿酒酵母COX2 mRNA的线粒体翻译受指定Pre-Cox2p前导肽的核苷酸序列控制。

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The mitochondrial gene encoding yeast cytochrome oxidase subunit II (Cox2p) specifies a precursor protein with a 15-amino-acid leader peptide. Deletion of the entire leader peptide coding region is known to block Cox2p accumulation posttranscriptionally. Here, we examined in vivo the role of the pre-Cox2p leader peptide and the mRNA sequence that encodes it in the expression of a mitochondrial reporter gene,ARG8m , fused to the 91st codon ofCOX2. We found within the coding sequence antagonistic elements that control translation: the positive element includes sequences in the first 14 codons specifying the leader peptide, while the negative element appears to be within codons 15 to 91. Partial deletions, point mutations, and local frameshifts within the leader peptide coding region were placed in both thecox2::ARG8m reporter and inCOX2 itself. Surprisingly, the mRNA sequence of the first six codons specifying the leader peptide plays an important role in positively controlling translation, while the amino acid sequence of the leader peptide itself is relatively unconstrained. Two mutations that partially block translation can be suppressed by nearby sequence substitutions that weaken a predicted stem structure and by overproduction of either the COX2 mRNA-specific translational activator Pet111p or the large-subunit mitochondrial ribosomal protein MrpL36p. We propose that regulatory elements embedded in the translated COX2 mRNA sequence could play a role, together with trans-acting factors, in coupling regulated synthesis of nascent pre-Cox2p to its insertion in the mitochondrial inner membrane.
机译：编码酵母细胞色素氧化酶亚基II（Cox2p）的线粒体基因指定了具有15个氨基酸前导肽的前体蛋白。已知整个前导肽编码区的缺失会在转录后阻断Cox2p的积累。在这里，我们在体内检查了前Cox2p前导肽及其编码的mRNA序列在融合到线粒体报道基因 ARG8 m 中的作用。 COX2 的第91个密码子。我们在编码序列中发现了控制翻译的拮抗元件：正向元件包括指定前导肽的前14个密码子中的序列，而负向元件似乎位于15到91之间的密码子中。部分缺失，点突变和局部移码前导肽编码区位于 cox2 :: ARG8 m 报告基因和 COX2 本身中。出乎意料的是，指定前导肽的前六个密码子的mRNA序列在正向控制翻译中起重要作用，而前导肽本身的氨基酸序列相对不受限制。可以部分削弱翻译功能的两个突变可以被附近的序列取代所抑制，这些突变会削弱预测的茎结构，并产生过量的 COX2 mRNA特异性翻译激活因子Pet111p或线粒体大亚基核糖体蛋白MrpL36p。我们建议，嵌入在翻译后的 COX2 mRNA序列中的调控元件可以与 trans 作用因子一起，在新生的pre-Cox2p的插入调控合成中发挥作用。在线粒体内膜中。

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