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首页> 外文期刊>Molecular and Cellular Biology >Stage-specific synthesis of proteins complexed to ribonucleoprotein particles and ribosomes in zoospores of Blastocladiella emersonii.
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Stage-specific synthesis of proteins complexed to ribonucleoprotein particles and ribosomes in zoospores of Blastocladiella emersonii.

机译：阶段性合成的蛋白质与核糖核蛋白颗粒和核糖体在埃弗氏杆菌的游动孢子中复合。

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摘要

In Blastocladiella emersonii zoospores, a set of proteins was found associated with the ribosomes and free ribonucleoprotein particles distinct from the ribosomes and polyribosomes. These proteins were designated P120, P105, P64, P56, and P42 based on their molecular weights determined by gel electrophoresis. Synthesis of these proteins was detected only during late sporulation just before the time polyadenylated ribonucleic acid accumulates in the sporangia. These proteins banded in isopycnic metrizamide gradients at densities of 1.31 and 1.27 g/cm3, which corresponded to the densities of the ribosomes and free ribonucleoprotein particles, respectively. Comparison of the distribution of the proteins in sucrose versus metrizamide gradients suggested that P105 was removed from the free ribonucleoprotein particles before complexing with the ribosomes. During germination, these proteins disappeared from the ribosomal fractions, with kinetics corresponding to the resumption of protein synthesis. Another protein (P178) was observed to bind to the ribosomes before the onset of protein synthesis during germination. Cycloheximide did not block the addition of this protein to the monoribosomes.
机译：在艾默生小球藻的游动孢子中，发现一组蛋白质与核糖体和游离的核糖核蛋白颗粒（不同于核糖体和多核糖体）有关。根据通过凝胶电泳测定的分子量，将这些蛋白质命名为P120，P105，P64，P56和P42。仅在聚腺苷酸化核糖核酸在孢子囊中积聚之前的后期孢子形成期间才检测到这些蛋白质的合成。这些蛋白以1.31和1.27 g / cm3的等密度甲氨riz呤梯度谱带，分别对应于核糖体和游离核糖核蛋白颗粒的密度。蔗糖和氨甲酰胺梯度中蛋白质分布的比较表明，P105在与核糖体复合之前先从游离的核糖核蛋白颗粒中去除。在发芽过程中，这些蛋白质从核糖体组分中消失，其动力学与蛋白质合成的恢复相对应。观察到另一种蛋白质（P178）在发芽过程中蛋白质合成开始之前与核糖体结合。环己酰亚胺没有阻止这种蛋白质添加到单核糖体中。

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