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首页> 外文期刊>Molecular and Cellular Biology >Sporulation and rna2 lower ribosomal protein mRNA levels by different mechanisms in Saccharomyces cerevisiae.
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Sporulation and rna2 lower ribosomal protein mRNA levels by different mechanisms in Saccharomyces cerevisiae.

机译：酿酒酵母中孢子形成和rna2降低核糖体蛋白mRNA水平的机制不同。

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In Saccharomyces cerevisiae, the levels of ribosomal protein mRNAs are regulated coordinately. Vegetative strains carrying the temperature-sensitive rna2 mutation exhibit a dramatic decrease in the levels of most ribosomal protein mRNAs at the restrictive temperature. Similarly, in wild-type cells induced to sporulate by nitrogen starvation, there is a fivefold reduction in the relative synthesis rate of ribosomal proteins. Using Northern gel analysis and cloned ribosomal protein genes, we compared the way in which ribosomal protein mRNA is affected under these two conditions. In vegetative rna2 cells, incubation at 34 degrees C led to the disappearance of ribosomal protein mRNAs and the accumulation of higher-molecular-weight precursor RNAs. A different phenotype was observed during sporulation. Although sporulating conditions led to a significant reduction in the relative abundance of ribosomal protein mRNA, there was no detectable accumulation of precursor RNAs even in rna2/rna2 diploids at 34 degrees C. A suppressor of rna2 and of other rna mutations, SRN1, at least partially relieved the block in the splicing of the ribosomal protein 51 intron in vegetative rna2 cells but did not detectably affect the level of ribosomal protein mRNA in sporulating cells. We concluded that the rna2 mutation and sporulation conditions affected ribosomal protein mRNA metabolism in two quite different ways. In vegetative cells the mutant rna2 effected a block which occurred primarily in post-transcriptional processing, whereas in sporulating cells the ribosomal protein mRNA levels were decreased by some other mechanism, presumably a change in the relative rate of transcription or mRNA turnover. Furthermore, the data suggest that the mutation rna2 has no additional effect on ribosomal protein mRNA metabolism in sporulating cells.
机译：在酿酒酵母中，核糖体蛋白mRNA的水平受到协调调节。携带温度敏感rna2突变的营养菌株在限制温度下大多数核糖体蛋白mRNA的表达水平显着下降。类似地，在氮饥饿诱导形成孢子的野生型细胞中，核糖体蛋白的相对合成速率降低了五倍。使用Northern凝胶分析和克隆的核糖体蛋白基因，我们比较了在这两种情况下核糖体蛋白mRNA受到影响的方式。在营养性rna2细胞中，在34摄氏度下孵育会导致核糖体蛋白mRNA的消失和高分子量前体RNA的积累。在孢子形成过程中观察到不同的表型。尽管形成孢子的条件导致核糖体蛋白mRNA的相对丰度大大降低，但即使在34°C的rna2 / rna2二倍体中也没有可检测到的前体RNA积累。rna2和其他rna突变的抑制剂SRN1，至少部分缓解了营养性rna2细胞中核糖体蛋白51内含子剪接的阻滞，但并未检测到影响孢子形成细胞中核糖体蛋白mRNA的水平。我们得出的结论是，rna2突变和孢子形成条件以两种完全不同的方式影响核糖体蛋白mRNA的代谢。在营养细胞中，突变体rna2产生了一个主要在转录后加工中发生的阻滞作用，而在孢子形成细胞中，核糖体蛋白mRNA的水平则通过其他一些机制而降低，大概是相对转录率或mRNA转化率发生了变化。此外，数据表明，突变rna2对孢子形成细胞中的核糖体蛋白mRNA代谢没有其他影响。

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