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首页> 外文期刊>Molecular and Cellular Biology >Selective linkage disruption in human-Chinese hamster cell hybrids: deletion mapping of the leuS, hexB, emtB, and chr genes on human chromosome 5.
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Selective linkage disruption in human-Chinese hamster cell hybrids: deletion mapping of the leuS, hexB, emtB, and chr genes on human chromosome 5.

机译：人-中国仓鼠细胞杂种中的选择性连锁破坏：人类染色体5上leuS，hexB，emtB和chr基因的缺失图谱。

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摘要

Chinese hamster-human interspecific hybrid cells, which contain human chromosome 5 and express four genes linked on that chromosome, were subjected to selective conditions requiring them to retain one of the four linked genes, leuS (encoding leucyl-tRNA synthetase), but lose another, either emtB (encoding ribosomal protein S14) or chr. Cytogenetic and biochemical analyses of spontaneous segregants isolated by using these unique selective pressures have enabled us to determine the order and regional location of the leuS, hexB, emtB, and chr genes on human chromosome 5. These segregants arise primarily by terminal deletions of various portions of the long arm of chromosome 5. Our results indicate that the order of at least three of these genes is the same on human chromosome 5 and Chinese hamster chromosome 2. Thus, there appears to be extensive homology between Chinese hamster chromosome 2 and human chromosome 5, which represents an extreme example of the conservation of gene organization between very divergent mammalian species. In addition, these hybrids and selective conditions provide a very simple and quantitative means to assess the potency of various agents suspected of inducing gross chromosomal damage.
机译：中国仓鼠-人类种间杂种细胞包含人类5号染色体并表达在该染色体上连接的四个基因，处于选择性条件下，要求它们保留四个连接的基因之一leuS（编码亮氨酰tRNA合成酶），但会丢失另一个，即emtB（编码核糖体蛋白S14）或chr。通过使用这些独特的选择压力分离的自发分离子的细胞遗传学和生化分析，使我们能够确定leuS，hexB，emtB和chr基因在人类5号染色体上的顺序和区域位置。这些分离子主要是由于各个部分的末端缺失而产生的结果表明，这些基因中至少三个的顺序在人类5号染色体和中国仓鼠2号染色体上是相同的。因此，中国仓鼠2号染色体与人类染色体之间似乎存在广泛的同源性。图5代表了在非常不同的哺乳动物物种之间保存基因组织的极端例子。另外，这些杂种和选择性条件提供了一种非常简单和定量的方法来评估怀疑诱导总染色体损伤的各种试剂的效力。

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