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首页> 外文期刊>Molecular and Cellular Biology >Organization of the noncontiguous promoter components of adenovirus VAI RNA gene is strikingly similar to that of eucaryotic tRNA genes.
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Organization of the noncontiguous promoter components of adenovirus VAI RNA gene is strikingly similar to that of eucaryotic tRNA genes.

机译：腺病毒VAI RNA基因的非连续启动子成分的组织与真核tRNA基因的组织极其相似。

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The intragenic transcriptional control region (internal promoter) of the adenovirus type 2 VAI RNA gene was mutated by deletion, insertion, and substitution of DNA sequences at the plasmid level. The mutant plasmids were assayed for in vitro transcriptional activity by using HeLa cell extracts. The mutant clones with substitution or insertion of DNA sequences or both between nucleotides +18 and +53 of the VAI RNA gene were all transcriptionally active, although to various extents. Substitution of unrelated DNA sequences up to +26 or between +54 and +61 abolished the transcriptional activity completely. Based on these results, the intragenic promoter sequences of the VAI RNA gene can be subdivided into two components: element A, +10 to +18; and element B, +54 to +69. The distance between the A and B components could be enlarged from its normal 35 base pairs to 75 base pairs without destroying the transcriptional activity. However, a deletion of 4 or 6 base pairs in the DNA segment separating the A and B components (segment C) reduced the transcriptional activity of the genes to less than 2% of that of the wild type. When the VAI RNA gene with its element A or B was substituted for the corresponding element A or B of the Xenopus laevis tRNAMet gene, the hybrid genes transcribed close to the level of the wild-type VAI RNA gene and about 10- to 20-fold more efficiently than the tRNAMet gene. Thus, the organization of DNA sequences in the internal promoter of the VAI RNA gene appears to be very similar to that of eucaryotic tRNA genes. This similarity suggests an evolutionary relationship of the VAI RNA gene to tRNA genes.
机译：通过缺失，插入和取代质粒水平的DNA序列使2型腺病毒VAI RNA基因的基因内转录控制区（内部启动子）发生突变。使用HeLa细胞提取物检测突变质粒的体外转录活性。尽管在不同程度上，在VAI RNA基因的核苷酸+18和+53之间具有DNA序列的替换或插入或二者均存在的突变克隆均具有转录活性。最多+26或+54至+61的不相关DNA序列的取代完全消除了转录活性。基于这些结果，可以将VAI RNA基因的基因内启动子序列分为两个部分：元件A，+ 10至+18;元件A，+ 10至+18。和元素B，+ 54至+69。 A和B组件之间的距离可以从其正常的35个碱基对扩大到75个碱基对，而不会破坏转录活性。但是，在将A和B成分分开的DNA片段（片段C）中缺失4或6个碱基对，使基因的转录活性降低到野生型转录活性的2％以下。当用非洲爪蟾tRNAMet基因的相应元素A或B替换VAI RNA基因时，杂种基因的转录接近野生型VAI RNA基因的水平，大约10至20折叠比tRNAMet基因更有效。因此，在VAI RNA基因的内部启动子中的DNA序列的组织似乎与真核tRNA基因的组织非常相似。这种相似性暗示了VAI RNA基因与tRNA基因的进化关系。

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