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首页> 外文期刊>Molecular and Cellular Biology >In vivo levels of diadenosine tetraphosphate and adenosine tetraphospho-guanosine in Physarum polycephalum during the cell cycle and oxidative stress.
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In vivo levels of diadenosine tetraphosphate and adenosine tetraphospho-guanosine in Physarum polycephalum during the cell cycle and oxidative stress.

机译：在细胞周期和氧化应激过程中，cephal头草中的尿苷四磷酸和腺苷四磷酸-鸟苷水平。

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Cellular levels of diadenosine tetraphosphate (Ap4A) and adenosine tetraphospho-guanosine (Ap4G) were specifically measured during the cell cycle of Physarum polycephalum by a high-pressure liquid chromatographic method. Ap4A was also measured indirectly by a coupled phosphodiesterase-luciferase assay. No cell cycle-specific changes in either Ap4A or Ap4G were detected in experiments involving different methods of assay, different strains of P. polycephalum, or different methods of fixation of macroplasmodia. Our results on Ap4A are in contrast with those reported previously (C. Weinmann-Dorsch, G. Pierron, R. Wick, H. Sauer, and F. Grummt, Exp. Cell Res. 155:171-177, 1984). Weinmann-Dorsch et al. reported an 8- to 30-fold increase in Ap4A in early S phase in P. polycephalum, as measured by the phosphodiesterase-luciferase assay. We also measured levels of Ap4A, Ap4G, and ATP in macroplasmodia treated with 0.1 mM dinitrophenol. Ap4A and Ap4G transiently increased three- to sevenfold after 1 h and then decreased concomitantly with an 80% decrease in the level of ATP after 2 h in the presence of dinitrophenol. These results do not support the hypothesis that Ap4A is a positive pleiotypic activator that modulates DNA replication, but they are consistent with the hypothesis proposed for procaryotes that Ap4A and Ap4G are signal nucleotides or alarmones of oxidative stress (B.R. Bochner, P.C. Lee, S.W. Wilson, C.W. Cutler, and B.N. Ames, Cell 37:225-232, 1984).
机译：通过高压液相色谱法在poly头cephal的细胞周期中专门测量了四磷酸腺苷四磷酸（Ap4A）和腺苷四磷酸鸟苷（Ap4G）的水平。还通过偶联的磷酸二酯酶-荧光素酶测定法间接测量了Ap4A。在涉及不同测定方法，不同头孢青霉菌株或不同固定大质粒方法的实验中，未检测到Ap4A或Ap4G的细胞周期特异性变化。我们在Ap4A上的结果与先前报道的结果相反（C. Weinmann-Dorsch，G。Pierron，R。Wick，H。Sauer和F. Grummt，Exp。Cell Res。155：171-177，1984）。 Weinmann-Dorsch等。据报道，通过磷酸二酯酶-荧光素酶测定，多头疟原虫的早期S期Ap4A增加了8至30倍。我们还测量了用0.1 mM二硝基苯酚处理的大质粒中Ap4A，Ap4G和ATP的水平。 1h后，Ap4A和Ap4G瞬时增加了三到七倍，然后在存在二硝基苯酚的情况下，在2小时后，ATP的含量下降了80％。这些结果不支持Ap4A是调节DNA复制的正性多型激活因子的假说，但与原核生物提出的Ap4A和Ap4G是氧化应激的信号核苷酸或警报蛋白的假说一致（BR Bochner，PC Lee，SW Wilson） ，CW Cutler和BN Ames，Cell 37：225-232，1984）。

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