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首页> 外文期刊>Molecular and Cellular Biology >Somatic excision of transposable element Tc1 from the Bristol genome of Caenorhabditis elegans.
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Somatic excision of transposable element Tc1 from the Bristol genome of Caenorhabditis elegans.

机译：秀丽隐杆线虫的布里斯托尔基因组的转座因子Tc1的体细胞切除。

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We investigated the ability of the transposable element Tc1 to excise from the genome of the nematode Caenorhabditis elegans var. Bristol N2. Our results show that in the standard lab strain (Bristol), Tc1 excision occurred at a high frequency, comparable to that seen in the closely related Bergerac strain BO. We examined excision in the following way. We used a unique sequence flanking probe (pCeh29) to investigate the excision of Tc1s situated in the same location in both strains. Evidence of high-frequency excision from the genomes of both strains was observed. The Tc1s used in the first approach, although present in the same location in both genomes, were not known to be identical. Thus, a second approach was taken, which involved the genetic manipulation of a BO variant, Tc1(Hin). The ability of this BO Tc1(Hin) to excise was retained after its introduction into the N2 genome. Thus, we conclude that excision of Tc1 from the Bristol genome occurs at a high frequency and is comparable to that of Tc1 excision from the Bergerac genome. We showed that many Tc1 elements in N2 were apparently functionally intact and were capable of somatic excision. Even so, N2 Tc1s were prevented from exhibiting the high level of heritable transposition displayed by BO elements. We suggest that Bristol Tc1 elements have the ability to transpose but that transposition is heavily repressed in the gonadal tissue.
机译：我们研究了转座因子Tc1从线虫秀丽隐杆线虫变种的基因组中切除的能力。布里斯托尔N2。我们的结果表明，在标准实验室菌株（Bristol）中，Tc1切除的发生频率很高，与密切相关的Bergerac菌株BO中观察到的频率相当。我们以以下方式检查了切除。我们使用独特的序列侧翼探针（pCeh29）来研究两种菌株中位于相同位置的Tc1的切除。观察到从两个菌株的基因组高频切除的证据。尽管第一种方法中使用的Tc1存在于两个基因组中的相同位置，但尚不相同。因此，采取了第二种方法，该方法涉及BO变体Tc1（Hin）的遗传操作。该BO Tc1（Hin）切除的能力在引入N2基因组后得以保留。因此，我们得出结论，从布里斯托尔基因组中切除Tc1的频率很高，并且与从伯杰拉克基因组中切除Tc1的频率相当。我们显示，N2中的许多Tc1元素在功能上显然是完整的，并且能够进行体细胞切除。即使这样，仍可以防止N2 Tc1表现出BO元素显示的高水平的遗传易位。我们建议布里斯托尔Tc1元素具有转座的能力，但在性腺组织中该转座受到严重抑制。

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