...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular and Cellular Biology >Unstable and stable CAD gene amplification: importance of flanking sequences and nuclear environment in gene amplification.
【24h】

Unstable and stable CAD gene amplification: importance of flanking sequences and nuclear environment in gene amplification.

机译：不稳定和稳定的CAD基因扩增：侧翼序列和核环境在基因扩增中的重要性。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We analyzed the amplification of the CAD gene in independently isolated N-(phosphonacetyl)-L-aspartate-resistant clones derived from single parental clones in two mouse cell lines. We report for the first time that the CAD gene is amplified unstably in mouse cells, that the degree of instability varies greatly between clones, and that minute chromosomes and highly unstable chromosomelike structures contain the amplified sequences. These data are most consistent with the idea that the amplified unit in each clone consists of different flanking DNA and that such differences engender amplified sequences with unequal stability. We also introduced the mouse chromosome containing the CAD gene into hamster cells by microcell-mediated chromosome transfer to determine whether the propensity for unstable extrachromosomal amplification of the mouse CAD gene would prevail in the hamster cell nuclear environment. We report that the mouse CAD gene was amplified stably in expanded chromosomal regions in each of seven hybrids that were analyzed. This observation is consistent with the idea that the nuclear environment influences whether mutants containing intra- or extrachromosomally amplified sequences will be isolated.
机译：我们分析了CAD基因在独立分离的N-（膦酰基乙酰基）-L-天冬氨酸耐药克隆中的CAD基因的扩增，该克隆源自两个小鼠细胞系中的单亲本克隆。我们首次报道了CAD基因在小鼠细胞中的扩增不稳定，克隆之间的不稳定性程度差异很大，微小的染色体和高度不稳定的染色体样结构均包含扩增的序列。这些数据与以下观点最一致：每个克隆中的扩增单位由不同的侧翼DNA组成，并且这种差异导致扩增的序列具有不平等的稳定性。我们还通过微细胞介导的染色体转移将包含CAD基因的小鼠染色体引入仓鼠细胞，以确定在仓鼠细胞核环境中是否会普遍存在不稳定的小鼠CAD基因染色体外扩增的趋势。我们报告说，在分析的七个杂种中的每一个中，在扩展的染色体区域中稳定扩增了小鼠CAD基因。该观察结果与核环境影响包含染色体内或染色体外扩增序列的突变体是否会被分离的想法是一致的。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号