首页> 外文期刊>Molecular and Cellular Biology >Identical genomic footprints of the adenovirus EIIa promoter are detected before and after EIa induction.
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Identical genomic footprints of the adenovirus EIIa promoter are detected before and after EIa induction.

机译:在EIa诱导之前和之后,检测到腺病毒EIIa启动子的相同基因组足迹。

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Genomic DNase I footprinting was used to compare specific protein binding to the adenovirus type 5 early, EIa-inducible, EIIa promoter. Identical protection patterns of the promoter region were observed whether EIIa transcription was undetectable or fully induced. These results suggest that EIa-mediated transcriptional induction does not increase binding of limiting transcription factors to the promoter but rather that transactivation results from the proper interactions between factors already bound to their cognate sequences.
机译:基因组DNA酶I足迹用于比较特异性蛋白与早期5种腺病毒的EIa诱导型EIIa启动子的结合。观察到启动子区域的相同保护模式,无论是否检测到或完全诱导了EIIa转录。这些结果表明,EIa介导的转录诱导不会增加限制性转录因子与启动子的结合,而是由已经与它们的同源序列结合的因子之间的适当相互作用导致反式激活。

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