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首页> 外文期刊>Molecular and Cellular Biology >Dissociation of c-fos induction from macrophage differentiation in human myeloid leukemic cell lines.
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Dissociation of c-fos induction from macrophage differentiation in human myeloid leukemic cell lines.

机译：在人类髓样白血病细胞系中巨噬细胞分化引起的c-fos诱导解离。

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Treatment of five human myeloid leukemic cell lines (KG1, ML3, HL-60, U-937, and HEL) with TPA was followed by macrophage differentiation and was accompanied by an early and transient increase in the mRNA level of c-fos proto-oncogene. The induction of c-fos was also observed in human cell lines K562 and K-Gla that did not respond to TPA with terminal macrophage differentiation. The treatment of HL-60 and U-937 cell lines with 1-oleoyl-2-acetylglycerol, a synthetic analog of diacylglycerol that, like TPA, stimulates protein kinase C activity, was followed by early and transient induction of c-fos mRNA in the absence of terminal macrophage differentiation. Finally, treatment of HL-60 with TPA in the presence of retinal, an inhibitor of protein kinase C, drastically reduced the induction of c-fos mRNA but had no effect on the terminal macrophage differentiation that is induced in this cell line by TPA. These results indicate that the induction of c-fos and terminal macrophage differentiation in response to TPA treatment can be dissociated in the in vitro models provided by human myeloid leukemic cell lines. Moreover, these findings suggest that the induction of c-fos is not only insufficient but may also be unnecessary for the differentiation along the monocyte-macrophage pathway.
机译：用TPA处理5种人类髓样白血病细胞系（KG1，ML3，HL-60，U-937和HEL）后，巨噬细胞分化，并伴随着c-fos原始mRNA的早期和短暂升高。癌基因。在人细胞系K562和K-Gla中也观察到了c-fos的诱导，这些细胞对末端巨噬细胞的分化没有反应，但对TPA没有反应。用1-油酰基-2-乙酰甘油（一种二酰基甘油的合成类似物，与TPA一样，刺激蛋白激酶C活性）处理HL-60和U-937细胞系，随后早期和短暂诱导c-fos mRNA的表达。终末巨噬细胞分化的缺乏。最后，在视网膜（一种蛋白激酶C的抑制剂）存在下，用TPA处理HL-60可以大大降低c-fos mRNA的诱导，但对TPA在该细胞系中诱导的终末巨噬细胞分化没有影响。这些结果表明，响应TPA处理的c-fos诱导和终末巨噬细胞分化可以在人类髓样白血病细胞系提供的体外模型中解离。此外，这些发现表明，对c-fos的诱导不仅不足，而且对于沿单核细胞-巨噬细胞途径的分化也可能是不必要的。

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