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首页> 外文期刊>Molecular and Cellular Biology >Two lck transcripts containing different 5' untranslated regions are present in T cells.
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Two lck transcripts containing different 5' untranslated regions are present in T cells.

机译：T细胞中存在两个含有不同5'非翻译区的lck转录物。

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摘要

p56lck is a new member of the src family of cellular tyrosine protein kinases. It is expressed constitutively at a low level in normal T cells and at an elevated level in the LSTRA and Thy19 Moloney murine leukemia virus-induced thymoma cell lines. It is possible that the expression of p56lck at an elevated level contributes to the transformation of these thymoma cells. The structure of the mRNAs encoding p56lck was examined by using an RNase protection assay. Both a chimeric lck mRNA containing the 5' untranslated region of Moloney virus mRNA and a normal lck mRNA were found in Thy19 and LSTRA cells. The chimeric lck transcript was 4- to 10-fold more abundant than the normal transcript. Transcription arising from a viral promoter is therefore responsible for the elevated levels of lck mRNA in these two cell lines. Surprisingly, uninfected murine T cells were also found to contain lck transcripts with differing 5' untranslated regions. One species of mRNA was colinear with the region of the chromosome just upstream of the initiation codon for p56lck. The other appeared to arise through splicing of an unidentified 5' untranslated exon to a sometimes cryptic splice acceptor just upstream of the region encoding p56lck. These data suggest that lck is expressed through the use of at least two different promoters. The promoters could be subject to different forms of regulation.
机译：p56lck是细胞酪氨酸蛋白激酶src家族的新成员。它在正常T细胞中以低水平组成性表达，在LSTRA和Thy19莫洛尼鼠白血病病毒诱导的胸腺瘤细胞系中以高水平表达。 p56lck的高水平表达可能有助于这些胸腺瘤细胞的转化。通过使用RNase保护测定法检查编码p56lck的mRNA的结构。在Thy19和LSTRA细胞中均发现了含有莫洛尼病毒mRNA 5'非翻译区的嵌合lck mRNA和正常lck mRNA。嵌合的lck转录物比正常转录物丰富4至10倍。因此，由病毒启动子引起的转录是导致这两种细胞系中lck mRNA水平升高的原因。令人惊讶地，还发现未感染的鼠T细胞含有具有不同5'非翻译区的lck转录物。一种mRNA与p56lck起始密码子上游的染色体区域共线。另一个似乎是通过将未识别的5'未翻译外显子剪接至编码p56lck的区域上游的有时是隐秘的剪接受体而引起的。这些数据表明，通过使用至少两个不同的启动子来表达lck。启动子可以受到不同形式的调节。

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