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首页> 外文期刊>Molecular and Cellular Biology >Heat shock response of Saccharomyces cerevisiae mutants altered in cyclic AMP-dependent protein phosphorylation.
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Heat shock response of Saccharomyces cerevisiae mutants altered in cyclic AMP-dependent protein phosphorylation.

机译：啤酒酵母突变体的热激反应在环AMP依赖蛋白磷酸化中改变。

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摘要

When Saccharomyces cerevisiae cells grown at 23 degrees C were transferred to 36 degrees C, they initiated synthesis of heat shock proteins, acquired thermotolerance to a lethal heat treatment given after the temperature shift, and arrested their growth transiently at the G1 phase of the cell division cycle. The bcy1 mutant which resulted in production of cyclic AMP (cAMP)-independent protein kinase did not synthesize the three heat shock proteins hsp72A, hsp72B, and hsp41 after the temperature shift. The bcy1 cells failed to acquire thermotolerance to the lethal heat treatment and were not arrested at the G1 phase after the temperature shift. In contrast, the cyr1-2 mutant, which produced a low level of cAMP, constitutively produced three heat shock proteins and four other proteins without the temperature shift and was resistant to the lethal heat treatment. The results suggest that a decrease in the level of cAMP-dependent protein phosphorylation results in the heat shock response, including elevated synthesis of three heat shock proteins, acquisition of thermotolerance, and transient arrest of the cell cycle.
机译：当将在23摄氏度下生长的酿酒酵母细胞转移到36摄氏度时，它们开始合成热激蛋白，获得了对温度变化后进行致命热处理的耐热性，并在细胞分裂的G1期短暂停止了其生长周期。 bcy1突变体导致产生不依赖环AMP（cAMP）的蛋白激酶，在温度变化后没有合成三种热激蛋白hsp72A，hsp72B和hsp41。 bcy1细胞未能获得对致命热处理的耐热性，并且在温度变化后也没有被阻滞在G1期。相反，产生低水平cAMP的cyr1-2突变体组成性地产生了三种热激蛋白和其他四种蛋白，而没有温度变化，并且可以抵抗致命的热处理。结果表明，cAMP依赖性蛋白磷酸化水平的降低会导致热休克反应，包括三种热休克蛋白的合成增加，耐热性的获得和细胞周期的短暂停滞。

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