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首页> 外文期刊>Molecular and Cellular Biology >Multiple basal elements of a human hsp70 promoter function differently in human and rodent cell lines.
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Multiple basal elements of a human hsp70 promoter function differently in human and rodent cell lines.

机译：人hsp70启动子的多个基础元件在人和啮齿动物细胞系中的功能不同。

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摘要

The human heat shock protein 70 (hsp70) gene is expressed constitutively in a wide variety of cells. Two separate promoter domains determine this basal level of hsp70 expression. The proximal domain is contained within 84 bases of the transcription initiation site and consists of three elements which appear to interact with the TATA factor(s) and CCAAT-box-binding transcription factor and SP1, respectively. The proximal domain is sufficient for near-maximal basal expression to rodent cell lines. The distal promoter domain consists of sequences upstream of -84 and is necessary in conjunction with the proximal domain for full basal expression in human cell lines. Although in BALB/c 3T3 cells the distal promoter domain plays little role in basal expression, it is functional as evidenced by the ability to compensate efficiently for mutations in the proximal CCAATC homology. The distal domain does not compensate as efficiently for proximal-domain mutations in HeLa cells. Basal expression of this human hsp70 promoter is, therefore, determined by multiple elements. Fewer elements are required for basal expression in rodent cell lines than in human cell lines, suggesting that there are significant differences between the rodent and human transcription apparatuses.
机译：人类热激蛋白70（hsp70）基因在多种细胞中组成性表达。两个单独的启动子结构域决定了hsp70表达的基础水平。近端域包含在转录起始位点的84个碱基之内，由三个元素组成，这三个元素似乎分别与TATA因子和CCAAT-box-binding转录因子和SP1相互作用。近端结构域足以使啮齿动物细胞系的基础表达接近最大。远端启动子结构域由-84上游的序列组成，与近端结构域结合在一起对于人类细胞系中的完整基础表达是必需的。尽管在BALB / c 3T3细胞中，远端启动子结构域在基础表达中起着很小的作用，但它可以有效地补偿近端CCAATC同源性的突变，从而发挥功能。远端域不能有效补偿HeLa细胞中的近端域突变。因此，该人hsp70启动子的基础表达由多种元件决定。与在人细胞系中相比，在啮齿动物细胞系中进行基础表达所需的元素更少，这表明在啮齿动物和人类转录装置之间存在显着差异。

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