Induction of neurite formation in PC12 cells by microinjection of proto-oncogenic Ha-ras protein preincubated with guanosine-5'-O-(3-thiotriphosphate).

T Satoh; S Nakamura; Y Kaziro

首页> 外文期刊>Molecular and Cellular Biology >Induction of neurite formation in PC12 cells by microinjection of proto-oncogenic Ha-ras protein preincubated with guanosine-5'-O-(3-thiotriphosphate).

【24h】

Induction of neurite formation in PC12 cells by microinjection of proto-oncogenic Ha-ras protein preincubated with guanosine-5'-O-(3-thiotriphosphate).

机译：通过微注射与鸟苷-5'-O-（3-硫代三磷酸）预孵育的原癌Ha-ras蛋白，诱导PC12细胞中神经突的形成。

获取原文

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

Rat pheochromocytoma (PC12) cells differentiate to neuronal cells in response to nerve growth factor. It has been shown that microinjection of oncogenic but not proto-oncogenic p21 protein induces morphological differentiation in PC12 cells (D. Bar-Sagi and J. R. Feramisco, Cell 42:841-848, 1985). In this paper we describe a recombinant human proto-oncogenic Ha-ras protein which can effectively induce neurite extension of PC12 cells when microinjected as a complex with guanosine-5'-O-(3-thiotriphosphate). The protein was found to be less effective when complexed with GTP. On the other hand, an oncogenic ras protein coinjected with guanosine-5'-O-(2-thiodiphosphate) was entirely inactive. These results indicate that the binary p21-GTP complex, but not the p21-GDP complex, is effective in inducing differentiation in PC12 cells, irrespective of the oncogenic or the proto-oncogenic protein.

机译：大鼠嗜铬细胞瘤（PC12）细胞响应神经生长因子而分化为神经元细胞。已经显示微注射致癌的而非原致癌的p21蛋白在PC12细胞中诱导形态分化（D.Bar-Sagi和J.R.Feramisco，Cell 42：841-848，1985）。在本文中，我们描述了一种重组人原癌基因Ha-ras蛋白，当与鸟苷5'-O-（3-硫代三磷酸）配合物显微注射时，可以有效诱导PC12细胞的神经突延伸。当与GTP复合时，发现该蛋白质的效果较差。另一方面，与鸟苷-5'-O-（2-硫代二磷酸）共注射的致癌性ras蛋白完全没有活性。这些结果表明，无论致癌蛋白或原致癌蛋白，二元p21-GTP复合物而不是p21-GDP复合物均能有效诱导PC12细胞分化。

著录项

来源
《Molecular and Cellular Biology》 |1987年第12期|共4页
作者
T Satoh; S Nakamura; Y Kaziro;
展开▼
作者单位

展开▼
收录信息
原文格式 PDF
正文语种
中图分类细胞生物学;
关键词

相似文献

外文文献
中文文献
专利

1. Intracellular application of guanosine-5'-O-(3-thiotriphosphate) induces exocytotic granule fusion in guinea pig eosinophils. [J] . O Nüsse, M Lindau, O Cromwell, The Journal of Experomental Medicine . 1990,第3期

机译：鸟苷5'-O-（3-硫代三磷酸）的细胞内应用诱导豚鼠嗜酸性粒细胞中胞吐颗粒融合。
2. Testing the in vivo role of protein kinase C and c-fos in neurite outgrowth by microinjection of antibodies into PC12 cells. [J] . J G Altin, R Wetts, K T Riabowol, Molecular biology of the cell . 1992,第3期

机译：通过将抗体显微注射到PC12细胞中来测试蛋白激酶C和c-fos在神经突增生中的体内作用。
3. Neurite outgrowth of PC12 cells by 4′-O-β-d-glucopyranosyl- 3′,4-Dimethoxychalcone from Brassica rapa L. 'hidabeni' was enhanced by pretreatment with p38MAPK inhibitor [J] . NishinaA., KimuraH., TsukagoshiH., Neurochemical research . 2013,第11期

机译：芥菜的4'-O-β-d-吡喃葡萄糖基-3'，4-二甲氧基查尔酮对PC12细胞的神经突生长。
4. Effects of low-intensity pulsed ultrasound on nerve growth factor-induced neurite outgrowth mainly through ERK-CREB pathway in PC12 cells [C] . Lu Zhao, Yi Feng, Hong Hu, IEEE International Ultrasonics Symposium . 2015

机译：低强度脉冲超声主要通过PC12细胞中的ERK-CREB途径对神经生长因子诱导的神经突生长的影响
5. Dynamic aspects of the neuronal intermediate filament (NIF) network studied by microinjection of biotinylated NIF proteins into living cells. [D] . Straube-West, Karen. 1984

机译：通过将生物素化的NIF蛋白显微注射到活细胞中来研究神经元中间丝（NIF）网络的动态方面。
6. Induction of neurite formation in PC12 cells by microinjection of proto-oncogenic Ha-ras protein preincubated with guanosine-5-O-(3-thiotriphosphate). [O] . T Satoh, S Nakamura, Y Kaziro 1987

机译：通过微注射与鸟苷-5-O-（3-硫代三磷酸）预孵育的原癌Ha-ras蛋白诱导PC12细胞中神经突形成。
7. Induction of neurite formation in PC12 cells by microinjection of proto-oncogenic Ha-ras protein preincubated with guanosine-5'-O-(3-thiotriphosphate). [O] . Satoh, T, Nakamura, S, Kaziro, Y 1987

机译：通过微注射与鸟苷-5'-O-（3-硫代三磷酸）预孵育的原致癌Ha-ras蛋白，诱导PC12细胞中神经突形成。

Induction of neurite formation in PC12 cells by microinjection of proto-oncogenic Ha-ras protein preincubated with guanosine-5'-O-(3-thiotriphosphate).

摘要

著录项

相似文献

相关主题

期刊订阅